摘要
本文报道了用液相法合成八肽胆囊收缩素(H-Asp-Tyr(SO_3H)-Met-Gly-Trp-Met-Asp-Phe-NH_2,ccK_8)。先分别合成C-端四肽和N-端四肽,然后缩合成八肽,并用温和的乙酰硫酸吡啶盐(PAS)法使其酪氨酸的酚羟基硫酯化。根据其氨基酸组成,层析行为和生物活性等证实产物为纯品。
In this paper the synthesis of the octapeptide Cholecystokinin, H-Asp-Tyr (SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2, by the method of liquid phase condensation was described. C-terminal tetrapeptide and N-terminal tetrapeptide have been synthesized first using the liquid phase method and then coupled successively. The phenolic hydroxl of Tyr residue in CCK-8 is sulfated by a mild reagent, pyridinium acetylsul-fate (PAS).A homogeneous synthetic CCK-8 was finally identificated with respect to its amino acid composition, chromatographic behavior and biological activity.
基金
国家自然科学基金
关键词
八肽胆囊收缩素
CCK-8
合成
液相法
Octapeptide cholecystokinin
Liquid-phase method
Pyridinium acetylsulfate
