摘要
为探索多肽类物质的酶联免疫测定方法，采用紫外线照射及戊二醛活化酶标板包被八肽胆囊收缩素（ＣＣＫ８），并以此为基础进行酶联免疫吸附试验。结果显示：该方法可使ＣＣＫ８稳固地结合于酶标板上；竞争性抑制实验中游离的ＣＣＫ８与固相ＣＣＫ８可与抗体发生竞争结合，并呈良好的定量关系。该方法简便、重复性及稳定性好（批内及批间变异系数分别为４７５％及７８０％），可用于ＣＣＫ相关肽初步的结构分析。
This study was undertaken to develop a method for assaying octapeptide cholecystokinin(CCK 8) using ELISA.The design of the method was based on the pretreatment of polystyrene microplates with ultraviolet irradiation and glutaraldehyde activation piror to coating.The results showed that this pretreatment let to stable attachment of CCK 8 to the solid phase.Competitive ELISA with CCK 8 as a competitor gave excellent quantitive relationship.Using this method we distinguished two kinds of CCK related peptides.This method proved to be simple,stable and reproducible;the intra and inter assay coefficients of variation were 4 75% and 7 80% respectively.
出处
《华西医科大学学报》
CSCD
1998年第2期220-223,共4页
Journal of West China University of Medical Sciences
关键词
脑肠肽
胆囊收缩素
包被
ELISA
Enzyme linked immunosorbent assay Cholecystokinin Coating
