摘要
目的研究外源性细胞因子调节Th1/Th2平衡对脑胶质瘤细胞增殖的影响。方法分别将IFN-γ+IL-4单抗或IL-4+IFN-γ单抗与脑胶质瘤细胞共同孵育,半定量RT-PCR方法观察Th1/Th2类细胞因子基因表达的变化,并用MTT方法检测其对脑胶质瘤细胞生长情况的影响。结果脑胶质瘤细胞Th2类细胞因子的表达明显强于Th1类(P<0.01),IFN-γ+IL-4单抗可以增强IFN-γ的表达,抑制脑胶质瘤细胞的增殖并呈剂量依赖性关系(P<0.01);IL-4+IFN-γ单抗则使Th2类细胞因子的表达有所增强,促进脑胶质瘤细胞的增殖,也呈剂量依赖性关系(P<0.01)。结论外源性的细胞因子或细胞因子单抗可以调节Th1/Th2平衡,逆转Th2优势,影响脑胶质瘤细胞的生长。
Objective To study the influence of adjustment of balance of Th1/Th2by external cytokines on proliferation of glioma cells.Methods The gene expressions of Th1/Th2type cytokines in C6,9L,U251and SHG44glioma cells were detected by semiquantitative reverse transcription polymerase chain reaction(RT-PCR).After the cells were induced with IFN-γ+IL-4McAb and IL-4+IFN-γMcAb respectively,we isolated the total RNA to proceed RT-PCR again.The evaluation of cell proliferation was pro ce eded by MTT assay method.Results There was obviously predominant expression of Th2type cytokines in glioma cell lines(P<0.01).The expression intensity of IFN-γwas improved in IF N-γ+IL-4McAb groups and Th2type cytokines were enhanced in IL-4+IFN-γMcAb groups.IFN-γand IL-4McAb could cause the switch from Th2to Th1,and could remarkably inhibit the proliferation of glioma cells in a dose-dependent way(P<0.01).On the other hand,IL-4and IFN-γMcAb could strengthen the switch of Th2,and might stimulate the glioma cell growth,also in a dose-dependent way(P<0.01).Conclusions There is a Th2preponderance in glioma cells.IFN-γand IL-4McAb could regulate the switch from Th2to Th0or Th1,and inhibit the proliferation of glioma cells.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2001年第6期599-602,共4页
Acta Academiae Medicinae Sinicae
基金
山东省"九五"科技攻关项目(鲁卫攻关08)资助
