摘要
目的 :探讨溶血卵磷脂 (LPC)对巨噬泡沫细胞胆固醇外流的影响 ,为动脉粥样硬化 (AS)的防治研究提供理论依据。方法 :分离培养小鼠腹腔巨噬细胞 ,密度梯度超速离心法分离人血浆低密度脂蛋白 (LDL) ,乙酰化后形成乙酰化低密度脂蛋白 (AcLDL) ,用AcLDL负载巨噬细胞使之形成巨噬泡沫细胞模型。用酶学荧光法检测细胞胆固醇外流 ,用乳酸脱氢酶 (LDH)检测试剂盒检测培养基中LDH活性来反映LPC的细胞毒性。结果 :LPC处理巨噬泡沫细胞 2 4h后 ,培养基中胆固醇含量明显高于对照组 (1 7- 4倍 ) ,细胞内胆固醇含量明显低于对照组。且LPC引起细胞胆固醇外流增加同时 ,培养基中LDH活性并没有明显差异。结论 :在 10 - 80 μmol/L剂量范围内 ,LPC可以剂量依赖性地促进巨噬泡沫细胞胆固醇外流 ,且这一效应与LPC的细胞毒性之间没有关系。
AIM: To explore the effect of lysophosphatidylcholine(LPC) on cholesterol efflux from macrophage foam cells, and to offer experimental evidence for research on prevention and treatment of atherosclerosis.METHODS: Peritoneal macrophages and LDL were seperated from mice and serum of healthy volunteers, respectively. The foam cells were derived from macrophages in the presence of Acylated LDL (AcLDL). Cholesterol efflux from cells and LDH activity were measured by enzymetic fluorometry and LDH kit, respectively.RESULTS: After incubated with LPC for 24 hours, cholesterol efflux from macrophage foam cells increased significantly compared to control, and cellular cholesterol was lower than that in control group. At the same time, medium LDH activity of LPC group was not increased obviously. CONCLUSION: Within the dosage of 10-80 μmol/L, LPC can promote cholesterol efflux from macrophage foam cells in a dose-dependent manner, and this effect has nothing to do with cytotoxity of LPC.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第1期28-31,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No .39870 32 6 )
