摘要
目的探讨松果体摘除对β-淀粉样蛋白(Aβ)神经毒性影响。方法SD大鼠随机分为松果体摘除组和假手术组,术后40d给Aβ1-40右侧海马注射,7d后标本用改进甲醇刚果红染色以及HE、TUNEL法检测海马凋亡细胞、SABC法检测凋亡相关基因Bax、Bcl-2表达。结果实验组海马细胞凋亡数比对照组增多(P<0.01),Aβ周围Bax表达比对照组增强(P<0.01);而Bcl-2在两组中表达无明显差别(P>0.05)。结论大鼠松果体摘除可以使Aβ1-40诱导海马细胞凋亡增多,与Bax表达增强有关。
Objective To investigate effects of pinealectomy on amyloid β protein neurotoxicity.Methods The rats were divided into two groups .Amyloid β protein 40 was injected into the right hippocampus of the rats in the both groups above,at fortieth days after operation.The brain tissues were stained by progressive Corge Red staining and the apoptotic cells were examined by HE staining and TUNEL method after injection seven days,while Bax,Bcl 2 protein expression of apoptosis related gene were also stuided by SABC method.Results The apoptotic cells in the experimental rats were much more than the ones in the control(P< 0.01).The Bax protein expression around Aβ were more strong than the one in the control(P< 0.05),but there was not difference between the two groups in Bal 2 protein expression(P >0.05).Conclusions Pinealectomy of rats resulted in more apoptotic cells in the hippocampus induced by Aβ1 40,which related to strong Bax expression.
出处
《中国临床康复》
CSCD
2002年第3期346-347,共2页
Chinese Journal of Clinical Rehabilitation
