摘要
目的:探索晶体囊袋内灌注丝裂霉素对兔晶体上皮细胞的抑制作用及对兔眼的毒性作用。方法:在兔晶状体超声乳化吸出术中,用0.2ml不同浓度的丝裂霉素（MitomycinC,MMC）（0.1mg/ml、0.2 mg/ml、0.4mg/ml）在晶体囊袋内进行水分离,使其直接短暂作用于晶体上皮细胞。术后随访2个月,观察比较用药组和对照组兔晶体后囊混浊、眼内压的变化等;并观察术后组织病理学和超微结构的变化。结果:临床观察显示,术后2周只有对照组眼出现后囊膜混浊（posterior capsule opacification,PCO）。术后4周用药组眼的PCO明显比对照组眼轻,且随药物浓度增高而减轻。中、低浓度组兔眼的术后炎症反应与对照组眼比较无明显差异。高浓度组兔眼术后早期出现轻微的毒性反应。组织病理学检查表明,对照组的晶体上皮增生较用药组明显。MMC可引起晶体上皮细胞变性,其变化程度与药物浓度有关。高浓度眼睫状体有炎症细胞浸润及少量出血。结论:MMC能有效地抑制晶体上皮细胞的增殖,其作用强度与药物的浓度相关。兔晶体囊袋内应用0.1～0.2mg/ml的药物浓度是防治后发性白内障安全有效的药物。眼科学报 2001;17:88～92。
Objective: To study the inhibitory effect of Mitomycin C applied by intracapsular infusion method on rabbit lens epithelial cells and its intraocular toxicity. Methods: 0. 2 ml Mitomycin C with different concentration (0. 1 nig/ml, 0. 2 mg/ml, 0. 4 mg/ml) was injected into the capsularbags of the rabbits' lens during the hydrodissec-tion of phacoemulsification. The drug was exposed to the lens epithelial cells directly and transiently. In the two-month follow-up after the operation, the posterior capsule opacification and the change of intraocular pressure were observed and compared between the control group and the experimental group; the histopathologic and the ultrastruc-tural changes were also observed.Results: For clinical study in the rabbit, the posterior capsule opacification only appeared in the control group two weeks after the operation. Four weeks after the operation, the posterior capsular opacification in the eyes of experimental group was not so obvious as that of the control group. The higher the concentration of the drugs, the less the posterior capsule opacification was shown. There was no obvious difference in the postoperative inflammation between the control group and the low, middle-concentration experimental group. Slight toxic reaction was shown on the rabbits' eyes of the high-concentration experimental group in the early stage after the operation. Thehistopathologic examination showed that the proliferation of lens epithelial cells in the control group was more obvious than that in the experimental group. Mitomycin C could cause the degeneration of lens epithelial cells. The degree of degeneration was related to the drug' s concentration. The ciliary bodies of the rabbits' eyes in the high-concentration experimental group were infiltrated by inflammatory cells and bled slightly. Conclusion: Mitomycin C can inhibit the proliferation of lens epithelial cells effectively. Its intensity is related to the drug' s concentration. It' s a safe and effective drug in the prevention of after-catara
出处
《眼科学报》
2001年第2期88-92,共5页
Eye Science
基金
1997年广东省科委省重点攻关项目资助(编号48)
