摘要
目的 :探讨双价痢疾菌苗滴鼻免疫后对小鼠不同粘膜部位和系统免疫部位的影响。方法 :BALB/c小鼠随机分为3组 ,每组 2 0只 ,FSM 2 117或FS 5 416 4× 10 7CFU/只经滴鼻途径免疫小鼠 ,间隔 2w ,3次免疫后 7d活杀 ,分离NALT、鼻通道、脾、小肠PP及MLN淋巴细胞 ,采用流式细胞术检测其淋巴细胞表型的变化 ;收集鼻咽、肺、肠、生殖道冲洗液和血清 ,采用ELISA法检测其中特异性抗福氏、宋内氏LPSIgA和IgG。结果 :两株菌苗经鼻内免疫都诱发了鼻咽、肺、胃肠道和生殖道等不同粘膜部位及血清中特异性抗福氏、宋内氏LPSIgA、IgG的显著增加 (P <0 0 1) ;NALT、NP和PP淋巴细胞中CD3+ T细胞显著增加 ,其中以CD4+ T细胞增加为主 ;FSM 2 117免疫组的脾细胞中B2 2 0 + 细胞显著增加 ,而FS 5 416免疫组的脾细胞中CD3+ T细胞显著增加。结论 :两株菌苗经鼻粘膜免疫均可诱导不同粘膜部位及系统免疫反应的发生 ,鼻粘膜是一个安全有效的免疫途径。
Objective:To observe the effect on different mucosal sites and system immune sites after intranasal immunization with bivalent Shigella vaccines Methods:BALB/c mice were divided into three groups at random , 10 mice per group Mice were intranasally immunized respectively with FSM 2117or FS 5416 (4×10 7CFU) three doses with an interval of two weeks The NALT, NP, spleen, PP, MLN, lymphocytes were isolated on the seventh day after the last immunization to assay the change of the cell phenotype with FACS The nasal ?lung?intestine?genital tract lavage fluid and serum were taken to assay the specific IgA or IgG against F2a or Sonni LPS with ELISA Results:The specific IgA and IgG in different mucosal sites and serum increased significantly after intranasal immunization with two Shigella vaccines compared with the control (P<0 01) ; CD3 + T cells in NALT?NP?PP lymphocytes increased significantly ,in which CD4 + T cells increased mainly. B220 + cells of splenocytes increased in FSM 2117 group and CD3 + T cells of splenocytes increased in FS 5416 group Conclusion:Intranasal inoculation of two bivalent shigella vaccines can induce immune response in different mucosaal sites and systemic immune response , which is a safe and effective immunization route
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第10期521-524,共4页
Chinese Journal of Immunology
基金
国家863与973项目(No863 10 2 0 7 0 3 0 3
G1999 5 41 0 3 )
