摘要
目的研究不同佐剂与鼠疫F1-V融合重组蛋白抗原滴鼻免疫Balb/c小鼠,观察机体产生体液免疫和局部粘膜免疫反应的效果,为发展黏膜疫苗提供理论基础。方法鼠疫F1-V融合重组蛋白抗原按比例分别与PorB(2类外膜蛋白)重组蛋白、蛋白体佐剂制备黏膜疫苗,滴鼻免疫Balb/c小鼠3次,取尾静脉血,采用ELISA检测血清IgG及抗体亚型分类,并检测鼻咽、肺、小肠及阴道灌洗液sIgA;采用FAC检测脾淋巴细胞表型的变化。结果PorB重组蛋白佐剂疫苗组和蛋白体佐剂疫苗组较无佐剂组体液免疫抗体水平高、蛋白体佐剂疫苗组好于PorB重组蛋白佐剂疫苗组,但无显著性差异。结论PorB重组蛋白佐剂疫苗和蛋白体佐剂疫苗均能诱导较强的系统免疫和黏膜免疫应答,且PorB重组蛋白佐剂疫苗免疫效果可与蛋白体佐剂疫苗相媲美,可进一步论证是否可用PorB重组蛋白佐剂替代蛋白体佐剂,这为鼠疫粘膜疫苗的研制奠定了基础。
Objective To study the systemic and mucosal antibody response induced by intranasal administration of different adjuvants plague F1-V recombinant protein.Methods Plague F1-V was formulated with proteosome(Pro),and recombinant PorB(Porin B)protein.Balb/c mice were nasally immunized by the vaccine for 3 times and sacrificed in week 4.The specific IgG to plague in sera of the mice was detected by indirect ELISA;the specific sIgA to plague in nasal solution,lung solution,intestinal,and vaginal solution of the m...
出处
《免疫学杂志》
CAS
CSCD
北大核心
2009年第2期183-186,共4页
Immunological Journal
