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双价痢疾菌苗不同途径免疫小鼠对脾细胞及GALT中ASC影响的实验研究 被引量:1

Detection of ASC in spleen cells and GALT after immunization with two bivalent Shigella vaccines in different administration rout in mice
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摘要 目的:观察侵袭蛋白表达阴性(FSM-2117)和阳性(FS-5416)的两株双价痢疾菌苗经不同途径免疫小鼠后,脾细胞和粘膜相关淋巴组织(gut associated lymphoid tissues,GALT)中抗体分泌细胞(antibody secreting cell,ASC)的变化,以探讨免疫途径及侵袭蛋白表达与否对痢疾菌苗免疫效果的影响。方法: BALB/c小鼠随机分为 3组,每组 20只, FSM-2ll7或 FS-5416 4 x10^7CFU分别经滴鼻、灌胃及肠内注射3种途径免疫小鼠,间隔2w,第3次免疫后7d活杀分离脾、小肠pp及MIN淋巴细胞,采用BA-ELISASPOT法检测其中特异性抗福氏、宋内氏LPSIgA和IgG分泌细胞。结果:滴鼻途径及小肠内免疫都能够诱生脾细胞特异性IgA-ASC、IgG-ASC显著升高(P<0.01);仅滴鼻免疫组Ipa+株较Ipa-株升高显著(P<0.01)。滴鼻和小肠内免疫同时诱生肠GALT中派伊尔小结(Payer's patch,PP)和肠系膜淋巴结(mesenteric lymph node,MLN)淋巴细胞ASC显著升高(P<0.05,P<0.01),但株间比较无统计学差异。而同等剂量的灌胃免疫未能诱发脾细胞及GALT特异性ASC升高。结论:鼻粘膜和小肠内免疫可以有效地诱导脾脏和GALT淋巴细胞的免疫反应,且显著降低免疫原用量(本实验滴鼻剂量为常规灌胃的1/20),是一个安全有效的免疫途径。研? Abstract Objective:FSM-2117 and FS-5416 are two bivalent hybrid strains of S.flexneri and S.sonnei, constructed by this laboratory-The FS-5416 expresses four invassive plasmid antigens(IpaA、IpaB、IpaC and IpaD) and has a good contact heamolysis activity(CHA+ ), butFSM-2117 hasn' t. To observe the immunogenicity of Shigelk vaccines through three different mucosal administration rout, the changes of ASCin the spleen and Peyer's patch(PP) , mesenteric lymph nodes(MLN) lymphocytes are detected.Methods:BALB/c mice were divided intothree groups, 20 mice per group. Mice were immunized respectively with the Ipa+ or Ipa- vaccines(4 x 10~7 CFU) three times with an intervalof two weeks by intranasal、intragastric or intraintestinal administration routs. The spleen , PP , MLN lymphocytes were isolated of seventh dayat random after immunization. An BA-EIISASPOT was done to account the numbers of ASC.Results:The numbers of SIgA and SIgG ASC ofspleen , PP , MLN lymphocytes of intranasal and intraintestinal group were significantly increased . Significant difference were only observed inthe number of spleen lymphocytes SIgA and SIgG ASC of intranasal group between Ipa+ and Ipa- . Conclusion:Two bivalent Shigelk vaccinescan induce spleen and GALT immunity reaction by nasal or small intestinal mucosal in a low dosage compared with intragastric rout (about 1/20). Ipa can significantly increased the number of spleen lymphocytes SIgA and SIgG ASC of intranasal group.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2001年第12期633-635,639,共4页 Chinese Journal of Immunology
基金 国家863计划经费资助(课题号:l02-07-03-03)
关键词 痢疾菌苗 滴鼻免疫 肠内免疫 GALT ASC 小鼠 Bivalent Shigella vaccine Intranasal Inrtagastric/intraintestinal GALT ASC
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