摘要
Em基因的表达受ABA诱导 ,干旱和盐胁通过增加ABA含量或改变植物细胞对ABA的敏感性而诱导Em基因的表达。植物Em基因启动子存在 3个功能区 :5′远端AT富集区通过影响转录调节表达量 ,作用类似于非专一性增强子 ;ABA应答元件ABRE在ABA存在的情况下与转录因子EmBP 1相互作用能显著增强Em基因的表达 ;
Em gene is induced by ABA and environmental stresses such as desiccation and salt. The effect of desiccation and salt stresses on Em gene expression appears to operate through increasing the endogenous levels of ABA or increasing the sensitivity of cells to ABA. The Em promoter can be functionally dissected into three components. The 5'distal AT-rich region that is responsible for different levels of expression through effects on transcription. These sequences are analogous to nonspecific enhancer regions. The next region of regulatory sequences is the ABA response element (ABRE) that is responsible for a dramatic increase in expression in response to ABA as a result of an interaction between ABRE and EmBP-1. The third component is the 5'flanking region of the Em gene, which appears to be involved in the ultimate level of expression through posttranscription.
出处
《生物工程进展》
CAS
CSCD
2001年第6期77-80,共4页
Progress in Biotechnology
