摘要
为了研究杨树ABF2同源基因的表达规律,从毛果杨基因组DNA中克隆出Pt AREB1基因上游一段1 800 bp序列。序列分析结果表明,该序列含有逆境胁迫响应元件TC-rich repeats、ABA应答元件ABRE和茉莉酸甲酯(Methyl Jasmonate,Me JA)应答元件TGACG-motif等胁迫相关元件。在序列分析的基础上,构建了Pt AREB1基因启动子驱动GUS报告基因的植物表达载体,利用农杆菌介导的花粉管通道法获得转基因拟南芥。结果表明Pt AREB1启动子可以在干旱、ABA、盐、Me JA和SA胁迫下,驱动GUS基因在转基因拟南芥的根、茎和叶中表达。说明Pt AREB1基因可能与干旱、高盐等胁迫应答紧密相关。
In order to study the expression and regulation of ABF homologous gene in poplar,a 1 800 bp 5＇flanking sequence of PtA REB1 gene was isolated by PCR from genomic DNA of Populus trichocarpa. By promoter sequence analysis,the sequence contained stress-response element TC-rich,ABA-response element ABRE and MeJ A-response element TGACGmotif. By sequence analysis,the PtA REB1 promoter was fused to the GUS reporter gene to characterize its expression pattern in P. trichocarpa. From the Agrobacterium mediated transformation of Arabidopsis thaliana,the GUS gene was induced in Arabidopsis thaliana,and it expressed in roots,stems and leaves under ABA,drought,high salt,MeJ A and SA,suggesting the PtA REB1 promoter was responsive to ABA,drought and high salt stress.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2016年第4期18-24,共7页
Journal of Northeast Forestry University
基金
国家"863"计划重点项目(2013AA102704)
关键词
毛果杨
ABF转录因子
ABA
启动子
拟南芥
Populus trichocarpa
ABF transcription factor
ABA
Promoter
Arabidopsis thaliana
