摘要
目的 通过检测胃癌患者血清 型胶原、层粘连蛋白、纤维粘连蛋白含量的变化 ,探讨细胞外间质成分与胃癌浸润转移的关系。方法 分别采用放射免疫法及琼脂扩散法检测 2 0名正常人及 40例不同时期胃癌患者血清 型胶原、层粘连蛋白和纤维粘连蛋白的含量。结果 40例不同时期胃癌患者血清 型胶原、层粘连蛋白、纤维粘连蛋白含量水平明显高于正常人 ,P值分别 <0 .0 1、0 .0 1、0 .0 5。中晚期 (87.5 % )、低分化 (6 5 .0 % )、有淋巴结转移 (6 2 .5 % )胃癌组上述指标明显高于临床早期 (12 .5 % )、中高分化 (35 .0 % )、无淋巴结转移胃癌组 ,P均 <0 .0 5。不同组织类型胃癌组上述指标变化差异无显著性 ,P>0 .0 5。结论 不同时期胃癌患者存在着不同程度细胞外基质纤维组织的增生 ,其恶性程度的高低与间质增生程度有必然联系 ,细胞外基质纤维组织的增生一方面可为癌细胞的浸润生长提供依托 ,另一方面也反映了机体对抗和限制肿瘤转移的一种趋势。检测 型胶原、层粘连蛋白、纤维粘连蛋白血清含量变化有助于胃癌的诊断 ,并可作为临床判断胃癌恶性程度、淋巴转移趋势。
Objective To study the relationship between extracellular matrix and neoplastic metastasis by detecting the serum concentration changes of collagen Ⅳ,laminin,fibronectin in patients with gastric cancer.Methods Radioimmunoassy was used to determining the serum levels of collagen Ⅳ,laminin in 20 healthy students and 40 patients with gastric cancer,agar diffusion assay to fibronectin.Results The serum concentration of collagen Ⅳ,laminin,fibronectin in gastric cancer group was significantly higher than that in control group (P<0 01,0 01,0 05).In these groups of clinical late term (87.5%),poor histologic differentiation (65%),lymph node metastasis 62.5%,their level increased also remarkedly all (P<0.5).But there was not statistical significance in various histologic types (P>0.05).Conclusion There was a certain hyperplasia of extracellular matrix around gastric cancer tissue.The malignant level of gastric cancer is inevitably connected with hyperplastic degree of matrix.On the one hand,the hyperplastic fibrotic tissue of extracellular matrix can provide a support for the infiltrative growth of cancer cells,on the other hand,this can also reflect a tendency of resisting neoplastic metastasis in human body.Thus,it is inferred that the serum concentration changes of collagen Ⅳ,laminin,fibronectin can be viewed as a valuable parameter for evaluating malignant level and prognostic tendency of gastric cancer.
出处
《山西医药杂志》
CAS
2001年第6期482-484,共3页
Shanxi Medical Journal
