摘要
目的 研究人骨瞩间充质干细胞（Ｍｅｓｅｎｃｈｙｍａｌ ｓｔｅｍ ｃｅｌｌｓ，ＭＳＣｓ）的分化特性及其端粒酶活性。方法 取人胚胎股 骨骨瞩，分离、培养、扩增ＭＳＣｓ，原位杂交法检测ＭＳＣｓ的端粒酶活性。将ＭＳＣｓ种植于裸鼠皮下，４周后观察ＭＳＣｓ的 分化情况。结果人 ＭＳＣｓ呈端粒酶反应阳性。植入裸鼠体内后可分化成骨、软骨、脂肪、骨骼肌、肌腱样组织和无髓神经 纤维束样结构。结论人ＭＳＣｓ是一种具有多向分化能力的干细胞，具有较高的端粒酶活性。
Objective To study the differentiation of human mesenchymal stem cells (MSCs) derived from human fetal bone marrow and observe its telomerase activity. Methods MSCs were isolated from fetal bone marrow of the femur followed by cell culture and amplification. In situ hybridization was employed to detect telomerase activity in the MSCs. Subcutaneous implantation of MSCs into nude mice was performed to observe their differentiation potentials 4 weeks after the implantation. Results Human MSCs were found positive for telomerase activity and they showed the potential to differentiate into such tissue cells as of the bone, cartilage, adipose, skeletal muscle, tendon-like tissue and unmyelinated nerve fiber-like bundles. Conclusion Human MSCs are multipotent stem cells that may differentiate into many types of tissue cells with high levels of telomerase activity.
出处
《第一军医大学学报》
CSCD
北大核心
2001年第11期801-805,共5页
Journal of First Military Medical University
基金
国家重点基础研究发展规划项目(973)(G1999054308)
国家自然科学基金(30070368)
广州市科委科技攻关项
