摘要
对活性干酵母 ( ADY)的耐 Se、富 Se性能作了测试 ,并初步研究了利用 ADY摇瓶发酵生产富 Se酵母的培养条件 .结果表明 :ADY具有很强的耐 Se、富 Se能力 ;无机 Se添加量和培养时间等 ,是影响其 Se转化能力的主要因素 .在适宜的摇瓶发酵条件 (麦芽汁 1 2°Be,加 Se量1 5mg/L ,装量 60 m L/2 50 m L三角瓶 ,p H=6.0 ,30℃ ,30 h)下 ,富 Se酵母干重、有机 Se含量和 Se转化率分别可达 1 5.8g/L ,790 .0μg/g和 83.2 %
The selenium rich and selenium tolerant ability of active dry yeast(ADY) and its conditions of bioconversion of inorganic selenium to the organic seleniun were studied. The results showed that ADY has strong selenium rich and selenium tolerant ability and the culture conditions have a great influence on selenium rich ability.The concentraction of inorganic selenium in medium and culture time,etc., were main factors affecting bioconversion of inorganic selenium. Under the initial optimum conditions(the mash liquor of 12 °Be, the inorganic selenium concentraction of 15 mg/L,medium of 60 mL in a 250 mL flask, initial pH=6.0, and culture time 30 h at 30 ℃), the dry weight of selenium yeast and the content of organic selenium in the cells as well as the rate of se bioconversion were 15.8 g/L,790.0 μg/g and 83.2% in proper order.
出处
《郑州轻工业学院学报》
2001年第2期3-6,共4页
Journal of Zhengzhou Institute of Light Industry（Natural Science）
基金
河南省科技攻关项目! (豫科 97110 0 143
9810 70 0 16
