摘要
目的 探讨外源性神经生长因子 ( NGF)对癫痫发作大鼠海马神经细胞凋亡与野生型 P5 3 ( wt P5 3 )蛋白表达有无抑制作用。方法 采用 KA诱导癫痫发作大鼠模型。以 TUNEL方法标记 DNA片段 ,原位检测凋亡细胞 ;免疫组化 SP法检测 wt P5 3 蛋白。观察大鼠癫痫发作 4 0 h(注射 KA4 8h)后其海马 CA1神经细胞凋亡和 wt P5 3 蛋白表达情况及外源性 NGF对它们的影响。结果 大鼠急性癫痫发作后 ,其凋亡细胞和 wt P5 3 蛋白表达均明显增加 ,wt P5 3 蛋白表达与细胞凋亡呈正相关 ( r =0 .83 9,P <0 .0 1 ) ;给予 NGF,凋亡细胞数及 wt P5 3 表达均显著性降低 ( P<0 .0 1 )。结论 外源性 NGF对癫痫所致神经细胞凋亡有抑制作用 ,其抑制凋亡的分子机制可能是通过抑制 wt P5 3蛋白表达而实现的。
Objective To explore whether exogenous nerve growth factor (NGF) might inhibit the hippocampal neuronal apoptosis and wild\|type P 53 (wtP 53 ) protein expression induced by acute seizures or not Methods The model of kainic acid induced epilepsy was used,apoptotic cell death was identified with the terminal deoxynucleotidyl transferase mediated dUTP biotin in situ nick end labeling (TUNEL) technique to detect DNA fragmentation and wtP 53 protein expression was determined with immunohistochemistry(SP) After the seizures stopped for 40 h (kainic acid injections for 48 h),effects of NGF on the neuronal apoptosis and wtP 53 protein expression in the hippocampus of the rats were observed Results After acute seizures,apoptotic neurons and wtP 53 \|protein neurons all increased significantly Correlation analysis showed that neuronal apoptosis had positive correlation with wtP 53 protein expression (r=0 839,P<0 01) Injected NGF into the cerebral ventricle,the rats had less apoptosis neurons and wtP 53 protein neurons than those not injected NGF and killed at the same time (P<0 01) Conclusion Exogenous NGF can inhibit the hippocampal neuronal apoptosis induced by acute seizures,molecular mechanisms of which may be attributed to wtP 53 expression inhibited
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2001年第4期207-209,共3页
Journal of Apoplexy and Nervous Diseases
