摘要
目的 研究海人酸（ＫＡ）诱发癫痫后颗粒细胞轴突和树突发芽是否会引起齿状回内突触可塑性变化。方法 电镜下对海马齿状回分子层内突触的密度和突触连接面的弯曲形式等参数进行定量研究。结果１，ＫＡ注射后３ｄ，大鼠ＤＧ分子层内的突触数目明显减少；而ＫＡ注射后７ｄ，突触数目恢复到与对照组相似的状态。２．与对照组及ＫＡ庄射后３ｄ组相比，ＫＡ注射后７ｄ大鼠ＤＧ分子层内的突触中，笑型突触所占突触总数的比例显著减少，愁型突触所占突触总数的比例显著增加。结论 １．ＫＡ注射后 ７ｄ时 ＤＧ内颗粒细胞苔状纤维及树突发芽是功能性的。２．推测 ＫＡ注射后 ７ｄ时 ＤＧ内愁型突触增加的原因与发芽的苔状纤维释放谷氨酸增多有关。
Objective In order to investigate the synaptic p lasticity in dentate gyrus after seizures and axonal and dendrtic sprouting indu ced by KA administration.Methods The density of synapses,the c urvature forms of synaptic interface were studied under electron microscope.[ WT 5”HZ Results 1.The density of the synapse is decreased obviously 3 d ays af ter KA injection,while the density of synapses is increased to control level 7 d a ys after KA injection. 2.Compare with the curvation forms of synaptic interfac e of control animals and 3days after KA administration animals,the amount of smi le synapses is significantly decresed and the amount of frown synapses is signif icantly increased in the moleculous layer of d entate gyrus 7 days after KA injection.Conclusions 1.This re sult demonstrates that the axonal and dendritic sprouting of dentate granule cells is functional.2.The increase of frown synapse is related to the release of glutamate of sprouting mossy fibers.
出处
《解剖学报》
CAS
CSCD
北大核心
2000年第z1期121-123,共4页
Acta Anatomica Sinica
关键词
海人酸
癫痫
可塑性
突触
齿状回
Kainic acid
Epilepsy
Plasticity
Synapse
Dent ate gyrus
