摘要
目的 探讨茶多酚 (TP)减轻环孢素A(CsA)急性肾毒性的作用及其机制。方法 按照分组 ,分别给予大鼠橄榄油、TP、CsA及TP +CsA ,给药 14d后 ,取血测尿素氮及血、尿肌酐 ,计算肌酐清除率 ,以病理评分法记录肾脏损害程度 ,硫代巴比妥酸法 (TBA法 )测定大鼠肾组织的脂质过氧化水平 ,黄嘌呤氧化法测定肾组织超氧化物歧化酶 (SOD)的活力 ,二硫代二硝基苯甲酸 (DTNB)比色法测定谷胱甘肽过氧化物酶 (GSH Px)活力。结果 使用CsA后 14d ,大鼠的肾脏可见明显损害 ,肌酐清除率明显下降 ,组织丙二醛 (MDA)水平升高 ,SOD与GSH Px活性下降 ;TP具有减轻CsA肾毒性的作用。结论 茶多酚能有效地减轻CsA所致的大鼠肾脏毒性 ,可能与其清除氧自由基、保护肾组织中SOD及GSH Px活性有关。
Objective To investigate the protective effets of tea polyphenols (TP) on cyclosporine A (CsA) induced nephrotoxicity in rats and the mechanism. Methods The animals in 4 groups were respectively treated with olive oil, TP, CsA and TP plus CsA. After treatment for 14 days, all animals were sacrificed and blood was taken for measurements of urea nitrogen, serum and urine creatinine and creatinine clearance. The degree of renal injuries was graded by the pathological score. The renal tissue MDA value was measured by TBA method. The purine oxidation method was used to the determine the SOD activity of kidney. The glutathione peroxidase (GSH Px) activity of renal tissue was assayed by using DTNB method. Results CsA (15?mg·kg -1 ·d -1 , sci) induced severe renal damage, caused marked elevation in blood urea nitrogen and decreasement in creatinie clearance, increased renal MDA content, decreased renal SOD and GSH Px activities in rats. Administration of TP (80?mg·kg -1 ·d -1 , ig) significantly attentuated the nephrotoxicity induced by CsA. Conclusions TP could effectively attenuate the nephrotoxicity induced by CsA, which was contributed to the antioxidant properties of TP.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2001年第5期271-273,共3页
Chinese Journal of Organ Transplantation
