摘要
目的 研究急性运动性轴索型神经病（acute motor axonal neuropathy,AMAN）与HLA-Ⅰ类和Ⅱ类等位基因分型的关系,探讨AMAN患者免疫遗传的特点。方法 用改良快速盐析法抽提基因组DNA,采用聚合酶链反应-顺序特异性引物法（PCR-SSP）对33例AMAN患者和132例健康人进行HLA-Ⅰ类和Ⅱ类等位基因分型。结果AMAN组HLA-Ⅰ类基因中B15、B35频率增高,RR值分别为4.09和7.08。Pc（校正P值）分别为0.015和0.00081。AMAN组HLA-Ⅱ类基因中,虽然DR16的频率高于对照组（P=0.004）,但校正后的P值失去意义。结论HLA-B15、B35与AMAN的易感性可能有关联。未发现HLA-Ⅱ类基因与AMAN有明显的关联。
Objective To study the relationship between the susceptibility to acute motor axonal neuropathy(AMAN) and frequencies of HLA-class Ⅰ and Ⅱ alleles, and to investigate the characteristics of immune genetic of AMAN. Methods A case-control study was conducted on 33 AMAN and 132 healthy individuals. DNA was extracted from peripheral blood leucocytes by improved fast salt-out technique. HLA-class Ⅰ and Ⅱ gene were typied by DNA-based technology, PCR-sequence specific primers (PCR-SSP) method. Results The frequencies of HLA-B15[relative risk(RR) 4.09,PC 0.015] and HLA-35 (RR 7.08, PC 0.0008) of HLA-class Ⅰ were a significantly increased in patients with AMAN. Frequencies of HLA-DR16 of HLA-class Ⅱ was increased in patients with AMAN ( P = 0.004, but Pc> 0.05) . Conclusions HLA-B15 -B35 might be associated with susceptibility to AMAN. HLA- Ⅱ genes were not found to have any association with AMAN.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2001年第4期250-253,共4页
Chinese Journal of Nervous and Mental Diseases
基金
国家自然科学基金资助(编号:39470265)
河北省自然科学基金资助(编号:399351)
