摘要
用ApaⅠ等 15种识别 6碱基的限制性内切酶研究了蒙古羊、乌珠穆沁羊、湖羊和小尾寒羊线粒体DNA的RFLP。结果表明 ,在所研究的个体中共检测到 3 2个酶切位点 ,16种限制性态型 ,其中BglⅠ表现出多态 ,XhoⅠ无切点。 16种限制性态型可归结为 2种基因单倍型 ,即单倍型Ⅰ (BglⅠ A)、单倍型Ⅱ (BglⅠ B) ,单倍型Ⅰ为受试绵羊品种的基本单倍型。线粒体DNA多态度π值平均为 0 0 192 % ,表明受试绵羊品种线粒体DNA多态性比较贫乏。
WT5BZ]The RFLP of mitochondrial DNA (mtDNA) in several native sheep breeds was studied using fifteen restriction endonucleases recognizing 6 base pair,Apa Ⅰ,Bgl Ⅰ,Bgl Ⅱ,Cla Ⅰ,Dra Ⅰ,EcoR Ⅰ,EcoR Ⅴ,Hae Ⅱ,Hind Ⅲ,Kpn Ⅰ,Pst Ⅰ,Pvu Ⅱ,Sac Ⅰ,Sal Ⅰ,Sma Ⅰ and Xho Ⅰ.The results indicated that 32 restriction sites and 16 restriction morphs were detected which could be sorted into 2 haplotypes in all individuals,and Bgl Ⅰ was polymorphic.The haplotype Ⅰ (Bgl Ⅰ A) was the main haplotype.The average polymorphic degree ( π value=0.0192%) of mtDNA showed that the genetic diversity of mtDNA of tested sheep breeds was very poor. [WT5HZ]
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2001年第4期295-298,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
河北省教委博士基金资助项目
