摘要
对昆明地区 3种不同生产模式下的香石竹 (DianthuscaryophyllusL .)进行了调查 ,采集样本 146号 ,利用酶联免疫法和电镜检测法对样本感染香石竹病毒的情况进行检测。结果表明昆明地区主要流行的香石竹病毒为香石竹斑驳病毒和香石竹坏死斑点病毒。以带香石竹斑驳病毒的香石竹品种“俏新郎”为实验材料 ,研究了直接剥茎尖法、高温处理结合剥茎尖法和病毒痤处理结合剥茎尖法 3种方法在脱病毒效率和茎尖成苗率的差异。实验结果表明以加热处理结合剥茎尖法脱病毒效果最好 ,0 2mm茎尖脱病毒率可达 77 78% ,加 5 %病毒痤处理对脱病毒有一定的影响 ,直接剥茎尖法脱病毒效果最差。
Carnation( Dianthus caryophyllus )in 3 production models growing in Kunming was investigated, and 146 samples were collected for experiments. The samples were inspected by ELISA and SEM to identify whether they were infected by carnation virus. The results indicated the main prevalenting carnation viruses in Kunming region are carnation mottle virus and carnation necroflect virus. Using carnation variety 'Qiaoxinlang' as test material, which infected by carnation mottle virus, we studies the differences of de-virus rate and survive rate by using 3 different methods,i.e. direct meristem-tip culture, meristem-tip culture in combination with thermotherapy, and meristem-tip culture in combination with Ribavirini. The results indicated that meristem-tip culture in combination with thermotherapy has the best de-virus rate, and the de-virus rate of 0 2 mm meristem-tip reaches 77 78%, 5% Ribavirini has some effect on de-virus rate, and direct meristem-tip culture has lowest de-virus rate.
出处
《云南植物研究》
CSCD
北大核心
2001年第3期345-349,共5页
Acta Botanica Yunnanica
基金
中国科学院"西部之光"资助项目
中国科学院西南生物资源与生物多样性保护研究基地资助项目
