摘要
[目的]调查贵州省不同海拔地区马铃薯病害发生情况,鉴定出贵州省马铃薯易感病毒种类。[方法]采用双抗体夹心酶联免疫吸附测定法(DAS-ELISA)对在黔南州、黔东南州及毕节地区采集的805份马铃薯样品进行病毒检测。[结果]805份马铃薯样品中检出有29份马铃薯X病毒(PVX)为阳性;41份马铃薯Y病毒(PVY)为阳性;285份马铃薯S病毒(PVS)为阳性;23份马铃薯卷叶病毒(PL-RV)为阳性,带病毒率46.95%。[结论]对贵州马铃薯危害较重的病毒种类依次为:马铃薯S病毒(PVS)、马铃薯Y病毒(PVY)、马铃薯X病毒(PVX)、马铃薯卷叶病毒(PLRV)。
[ Objective ] The research aimed to investigate the situation of potato virus diseases' occurrence and identify the type of the potato susceptible virus at different altitudes in Guizhou Province. [ Method] 805 potato samples collected from Qiarman, Qiandongnan, Bijie Prefecture were detected by DAS-ELISA determination method. [ Result ] Among 805 potato samples, 29 samples were infected by potato virus X (PVX) , 41 samples were infected by potato virus Y (PVY) , 285 samples were infected by potato virus S (PVS) , 23 samples were infected by potato virus (PLRV). The virus-carrying rate was 46.95%. [ Conclusion] The virus ranking ( from most severe to least) affected potato in Guizhou was as follows: potato virus S(PVS) , potato virus Y (PVY), potato virus X (PVX), potato virus (PLRV).
出处
《安徽农业科学》
CAS
北大核心
2009年第29期14262-14263,共2页
Journal of Anhui Agricultural Sciences
基金
贵州省科学技术基金"贵州马铃薯易感病毒鉴定及防治技术研究"(黔科合J字[2006]2039号)
贵州省科技重大专项:"贵州省马铃薯产业化关键技术研究与示范之子项目1--贵州省马铃薯种质资源库建设"黔科合重大专项字(2008)6009
关键词
马铃薯
病毒病
调查
Potato
Virus disease
Investigation