摘要
研究了里氏木霉GXC产木聚糖酶的条件和酶学性质。结果表明，适宜产酶碳源为乳糖、甘露糖、棉子糖、木聚糖和麸皮，氮源为牛肉膏和酵母膏；产酶的最适初始pH为4.0，30℃培养60h。对以麸皮为碳源的培养液进行纯化的酶特性研究表明，木聚糖酶的最适反应温度为50℃，pH为5.5，该酶在pH5.0(7.0和40℃以下相对稳定。Fe3+和Mn2+对木聚糖酶有较大的促进作用，Cu～2+、Fe～2+和Ca～2+ 具有抑制作用。
The condition of xylanase production of Trichoderma reesei GXC and enzyme properties were studied. The suitable carbon and nitrogen sources for xylanase production were lactose, mannose, raffinose, xylan, wheat bran , beef extract and yeast extraction. The optimal condition for enzyme production was initial pH 4.0, shaking at 30℃ for 60 h. Xylanase was purified from the culture supernatant in the medium with wheat bran as carbon source.The optimal temperature and pH for purified xylanase reaction were 50℃ and 5.5, respectively. Xylanase was stable at pH 5.0～7.0 and below 40℃. The xylanase activity was significantly stimulated by Fe～3+ and Mn～2+, and inhibited by Cu～2+, Fe～2+ and Ca～2+.
出处
《菌物系统》
CSCD
北大核心
2001年第2期184-190,共7页
Mycosystema
基金
国家自然科学基金资助项目!(No. 30000118)
关键词
里氏木霉
木聚糖酶
产酶条件
特性
Trichoderma reesei, xylanase, condition of enzyme production, enzyme properties
