摘要
以玉米芯木聚糖为原料，里氏木霉（Ｔｒｉｃｈｏｄｅｒｍａｒｅｓｅｉ）ＲｕｔＣ３０为菌种，采用改进的Ｍａｎｄｅｌｓ配方制备木聚糖酶，产酶历程与制备纤维素酶时有较大差异。具体表现在产酶周期短，ｐＨ值基本无下降的趋势，酶液中可溶性蛋白质浓度较低。底物浓度为７ｇ／Ｌ时，木聚糖酶活力达１２５６ＩＵ／ｍＬ，比活力、酶得率及酶产率分别为８１９０ＩＵ／ｍｇ蛋白质、１７９４３ＩＵ／ｇ木聚糖和４１８６．７ＩＵ／Ｌ·ｄ。产酶最终ｐＨ应控制在６～７较为适宜，酶活力最高。ｐＨ超过７．０，酶可能失活。酶解结果表明，木聚糖酶对木聚糖干粉具有很高的降解效率，当每克底物的酶用量为０．１克木聚糖干粉产的酶液量时，酶解得率一般可达９０％左右。
The kinetic course of xylanase production by Trichoderma reesei Rut C30, using corncob xylan as the substrate, was different from that of cellulase production distinctly. The culture time of xylanase production was shorter than that of cellulase production. During cultivation, pH did not decline evidently and protein concentration kept at low levels throughout the cultivation period. In addition, the highest xylanase activity, up to 12.56 IU/mL, was obtained with 7 g/L xylan as the substrate. Specific activity and enzyme yield were 81.90 IU/mg and 1794.3 IU/g, respectively. Furthermore, it was important that the final pH value was controlled to 6.0～7.0 in order to obtain the highest activity, otherwise the enzyme activity would decline when the final pH value was exorbitant. The results of enzymatic hydrolysis have shown that the hydrolytic ability of xylanase produced by T. reesei was very effective to xylan. When a gram of xylan was treated with enzyme broth produced from 0.1 gram of xylan, the efficiency of enzymatic hydrolysis was about 90%. HPLC analysis indicated that the hydrolysate contained mainly monosaccharides.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
1998年第1期31-36,共6页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金
