摘要
目的 探讨胎盘肥胖基因及其蛋白———瘦素 (leptin)表达水平与脐血瘦素和胎儿宫内生长发育的关系。方法 40例产妇分娩时采集胎盘与脐血 ,其中 2 6例来自上海新华医院 ,14例来自南京江北人民医院。采用逆转录聚合酶链反应 (RT PCR)检测胎盘瘦素mRNA相对表达水平 ,采用Westernblot检测胎盘瘦素表达水平 ,采用免疫组织化学观察胎盘瘦素表达位点 ,采用放射免疫法(RIA)检测脐血瘦素水平 ,采用Ponderal指数 [PI=10 0×体重 (g) /身长 (cm) 3]估测新生儿营养状态。结果 RT PCR显示胎盘组织瘦素mRNA呈现高效表达 ,免疫组化显示胎盘滋养层细胞胞质内瘦素阳性表达。均数差别t检验显示 13例小于胎龄儿 (SGA)胎盘组织瘦素mRNA与 β actin mRNA表达水平之比为 0 6 1± 0 15 ,低于 15例适于胎龄儿 (AGA) 0 83± 0 2 0 (P =0 0 0 3 4) ,12例大于胎龄儿 (LGA)胎盘组织瘦素mRNA与 β actin mRNA表达水平之比为 1 0 0± 0 2 3,高于AGA(P =0 0 43)。 13例SGA胎盘组织瘦素含量 (ng/mg)为 0 2 6± 0 0 5 ,低于 15例AGA (0 340± 0 0 86 ) (P =0 0 0 76 ) ,12例LGA胎盘组织瘦素含量为 0 43± 0 10 ,高于AGA(P =0 0 2 1)。胎盘肥胖基因mRNA及其瘦素表达水平与脐血瘦素水平显著相关 (r =0 39和 0 43,P
Objective To study the role of placental leptin in intrauterine cord leptin production and its relationship with neonatal anthropometry. Methods Forty women and their babies were enrolled and approved by Xinhua Hospital (Shanghai, China) and Jiangbei Hospital (Nanjing, China) in this study. Placental tissue was assayed for leptin mRNA by reverse transcription/polymerase chain reaction (RT/PCR), and assayed for ob gene protein , leptin , by Western Blot and immunohistochemistry. Blood was taken from the umbilical cord of the babies at delivery. Serum leptin was measured by radio immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery. Linear regression analysis was used to explore the relationship between placental leptin, cord leptin and neonatal anthropometric measures. Results Ob gene was expressed in placental tissue at comparable or greater levels than that in adipose tissue. Comparison of the relative levels of leptin to β actin mRNA by multiplex RT/PCR revealed that the placenta of the small for gestational age (SGA) neonates expressed leptin mRNA at significantly lower levels 0 61±0 15 than that of the appropriate for gestational age (AGA) neonates 0 83±0 20 ( P =0 003 4), while the placenta of the large for gestational age (LGA) neonates expressed leptin mRNA at significantly higher levels 1 00±0 23 than that of the AGA neonates ( P =0 043). Immunohistochemical techniques showed the immunostaining pattern in the cytoplasm of trophoblastic cells. Western blot showed that the placenta of the SGA neonates expressed leptin at significantly lower levels 0 26±0 05 ng/mg than that of the AGA neonates 0 34±0 09 ng/mg ( P =0 007 6), while the placenta of the LGA neonates expressed leptin at significantly higher levels 0 43±0 10 ng/mg than that of the AGA neonates ( P =0 021). Linear regression analysis showed placental ob gene transcription and leptin translation correlated significantly with cord leptin ( r =0 39 and 0 43), an
出处
《中华医学杂志》
CAS
CSCD
北大核心
2001年第8期489-492,共4页
National Medical Journal of China
