摘要
浙江省 1996～ 1999年急性弛缓性麻痹 (AFP)病例粪便标本中非脊髓灰质炎 (脊灰 )肠道病毒 (NPEV)的平均分离率为 11 2 %。在 6 7株NPEV中 ,柯萨奇病毒 (Coxsackievirus ,Cox )B组 2 1株 ,占 31 3% ;埃可病毒 (EntricCy topothicHumanOrphanviruses ,ECHO) 2 5株 ,占 37 4% ;不能确定型别 2 1株 ,占 31 3%。NPEV按月分布显示 ,Cox B病毒高峰期在 5～ 7月 ,在 1～ 3、10、12月未分离到 ;ECHO病毒全年均有 ,高峰期在 8～ 11月。Cox B病毒在Hep - 2、RD细胞上平均滴度分别为 10 7 2 和 10 4 6TCID50 /ml,ECHO病毒则分别为 10 3 1和 10 6 9TCID50 /ml。部分不能确定型别的病毒除了能在RD、Hep - 2细胞生长外 ,还能在L2 0B细胞上生长 ,其细胞病变与“腺病毒”相类似 ,但与脊灰病毒在L2 0B细胞上的细胞病变效应明显不同。
An average isolation rate of non-polio enteroviruses (NPEV) was 11.2% in Zhejiang Province during 1996-1999. There were 21 strains of Coxsackie B (31.3%), 25 strains of ECHO (37.4%) and 21 strains of unclassified virus (31.3%) isolsted from a total of 67 NPEV. The monthly distribution of the NPEV isolated from 1996 to 1999 was determined. Coxsackie B virus activity peaked from May to July, but none of the Coxsackie B virus was isolated from January to March and from both October and December. ECHO were present throughout the year and peaked from August to November. The titers of Coxsackie B viruses in Hep-2 and RD cells were 10 7.2 TCID 50 /ml and 10 4.6 TCID 50 /ml, while ECHO were 10 3.1 TCID 50 /ml and 10 6.9 TCID 50 /ml, respectively. There were some unclassified viruses, which could grow in L20B cells, but the CPE was different from that of the poliovirus and was similar to adenovirus.
出处
《中国计划免疫》
2001年第2期82-85,共4页
Chinese Journal of Vaccines and Immunization
