摘要
应用紫外 可见吸收光谱法研究细胞色素C(Fecyt C)活性中心铁离子在pH 7 1的磷酸缓冲液中与 4种微量元素M(Ⅱ )的直接相互作用。结果表明 ,细胞色素C(Fecyt C)活性中心Fe(Ⅱ )离子可被微量元素M(Ⅱ )部分取代 ,形成部分的细胞色素C衍生物 (Mcyt C) ,溶液的吸收光谱的Soret带最大吸收峰发生蓝移 ,且α带与β带最大吸收峰相对峰强减弱。
The direct interaction between the metal ion in the active center of cytochrome C(Fecyt-C) and four trace elements in pH 7.1 phosphate buffer by UV spectrometry has been studied. As a result,part of the metal ion Fe(II) in Fecyt-C was replaced and the corresponding cytochrome C derivatives were produced. The Soret absorption maximum is blue-shifted and the absorption coefficient of the a band relative to that of the beta band is decreased in comparison to the Fecyt-C.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2001年第2期222-223,共2页
Spectroscopy and Spectral Analysis
