摘要
应用紫外 可见吸收光谱法研究细胞色素C(Fecyt C)活性中心铁离子在 pH 7 1的磷酸缓冲液中与无机CuSO4 的直接相互作用。结果发现 :CuSO4 中的Cu(Ⅱ )与Fecyt C中的Fe(Ⅱ )有交换作用 ,形成部分的Cucyt C ,并且随着外加CuSO4 量的增加 ,这种相互作用增强 ,首次证实了细胞色素C这种直接相互作用的存在。
The direct interaction between the metal ion in the active center of cytochrome C (Fecyt-C) and inorganic copper sulfate in pH 7.1 phosphate has been studied by UV spectrometry. As a result, part of the metal ion Fe(II) in Fecyt-C was replaced by Cu(II) and the corresponding cytochrome C derivatives Cucyt-C were produced, in addition, the interaction was increased with amount of copper sulfate added. The direct interaction between the CuSO4 and metal ion of cytochrome C was verified for the first time.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2001年第1期54-56,共3页
Spectroscopy and Spectral Analysis
