摘要
采用以甲壳胺为基质合成的ＣＸ—１型微载体，进行Ｖｅｒｏ细胞悬浮培养实验。结果表明，在１ｍｇ／ｍｌ甲壳胺微载体浓度下，使用ＲＰＭＩ１６４０培养基，添加１０％新生牛血清，３７℃培养３ｈ，传代Ｖｅｒｏ细胞能够快速贴附于微载体上。悬浮培养２４ｈ时，细胞浓度达到２．２６×１０５个／ｍｌ；培养７２ｈ时，细胞浓度达到６．８９×１０５个／ｍｌ；培养１２０ｈ时，细胞浓度达到７．１２×１０５个／ｍｌ。电镜观察表明细胞在微载体上长势良好。
In order to study the growth of vero cells on the chitosan-microcarrier, we use 1mg/ml microcarrier; RPMI 1640 medium and supplemented with 10% fetal bovine serum to culture vero cells at 37℃. After 3h, the cells could adhere to the microcarrier and proliferated on it. Stirred at 50 rpm to culture for 24h; 72h and 120h, the density of the cells was 2.26×10 5, 6.89×10 5, and 7.12×10 5cells/ml, respectively. Observed on scanning electron microscope the growth of the cells was in good morphology. It is expected that chitosan-microcarrier would be suitable for culturing Vero cells at high densities.
基金
山东省科委重点资助
