摘要
目的：建立柴芍安神解郁颗粒的质量控制标准。方法：采用薄层色谱法对制剂中的丹参、柴胡、石菖蒲3味药进行定性鉴别；采用高效液相色谱法对制剂中的丹酚酸B进行定量测定。结果：薄层色谱法可清晰鉴别丹参、柴胡和石菖蒲，且阴性对照无干扰；丹酚酸B在0．0518～0．5180μg范围内线性关系良好，相关系数r=0．9999，平均回收率为98．82％．RSD=1．27％。结论：本方法操作简单、专属性强、重现性好、数据准确，可用于疏肝安神的质量控制。
Objective ：To establish the quality control standard for Chaishao Anshen Jieyu granules. Methods：Thin- layer chroma- tography（TLC） was used to identify Salvia mihiorrhiza, Bupleurum chinense, and Rhizoma Acori graminei in Chaishao Anshen Jieyu granules. High - performance liquid chromatography was used to determine the content of salvianolic acid B. Results ： Salvia miltiorrhiza, Bupleurum chinense, and Rhizoma Acori graminei were well identified by TLC, and the negative control had no interference. Salvianolic acid B showed a good linear relationship within O. 0518 -0. 5180 μg（ r = 0. 9999）. The average recovery rate was 98.82% , with a relative standard deviation of 1.27%. Conclusion：This method has the advantages of easy operation, high specificity, high repeatability, and high accuracy and can be used for the quality control of Chaishao Anshen Jieyu granules.
出处
《湖南中医杂志》
2014年第6期151-153,共3页
Hunan Journal of Traditional Chinese Medicine
基金
广东省中医药局资助项目(编号:20131110)
关键词
柴芍安神解郁颗粒
薄层色谱法
高效液相色谱法
丹酚酸B
质量标准
thin - layer chromatography
high - performance liquid chromatography
Chaishao Anshen Jieyu granules
salvianolic acid B
content determination
