摘要
目的建立功劳木中盐酸小檗碱的含量测定的方法,为功劳木药材质量控制提供科学依据.方法用反相高效液相色谱法(RP-HPLC)测定,采用十八烷基硅胶键合硅胶柱Kromasil C18(250 mm×4.6 mm,5 μm),流动相为甲醇-乙腈-水(40:20:40)(含0.05 mol·L-1十二烷基硫酸钠及0.037 mol·L-1酒石酸);检测波长为346 nm;流速为1 mL·min-1;柱温为50 ℃.结果盐酸小檗碱在0.082～1.312 μg峰面积与其浓度线性关系良好(r2=0.999 9),平均回收率为98.2%,重复性试验RSD为1.62%(n=5).结论 RP-HPLC测定功劳木药材中盐酸小檗碱的含量,不仅操作简便、准确,而且重现性好.
OBJECTIVE To establish a method to determine the contents of berberine in Caulis Mahonia. METHODS RP-HPLC and Kromasil C_ 18(250 mm×4.6 mm,5 μm)column was used,methanol-acetonitrile-water(40∶20∶40) as mobile phase. The column temperature was 50 ℃ and the flow rate was 1 mL·min -1. The detection wavelength was 346 nm. RESULTS There was a good linearity with in 0.082～1.312 μg of acetylglutamide(r2=0.999 9).The average recovery was 98.2%,and the RSD was 1.62%(n=5). CONCLUSIONS The method is rapid,accurate and reliable.
出处
《中南药学》
CAS
2005年第2期99-100,共2页
Central South Pharmacy
