摘要
matK基因是陆地植物核心条码候选片段之一,文章通过克隆获得22个核桃品种matK基因近3'端长度为726 bp EST序列,运用序列分析技术探索matK在核桃种间关系研究中的价值。结果表明,在所测的matK基因的核苷酸序列中发生变异的核桃品种均属早实核桃类群,而晚实核桃类群无变异发生;推导氨基酸序列分析显示薄壳香由于发生突变,提前终止翻译而只编码173个氨基酸,而其他品种均编码234个氨基酸;采用近邻接树法和最大简约法构建的系统进化树显示,22个品种核桃分为5个组,其中扎343与其他21个品种有明显差异,8个新选育的品种中金薄丰1号较为突出;而辽核1号与西扶1号亲缘关系最近。对matK基因深入研究将为核桃种质资源保护、杂交育种及开发运用提供分子生物学依据。
matK gene is known as one of the core bar code segments for the candidated land plants. In this paper, we acquired the matK genes 726 bp EST which located in the 3＆#39; end from 22 walnut varieties and explored the value of the matK gene on establishing walnut interspecies relation by the sequence analysis. The results demonstrate that the nucleotide sequence of early bearing walnuts matK genes＆#39; mutation has been detected, but the later mature walnuts were opposite. Amino Acid Sequence Analysis indicated that nuthin shel had promotes premature translational-termination and generates 173 amino acids because of mutations, which was extensively different the normal products 234 amino acids. Neighbours pick Neighbor-Joining and Maxium Parsimony was used to construct the system of evolutionary tree and 22 walnut varieties were divided into five groups, we concluded that Zha343 had distinct differences with other 21 walnut varieties, Jinbofeng1 gained an advantage over the other varieties among the novel varieties and Liaohe1 and xifu1 were the closest cousin. In a word, the further research in matK genes provide the a molecular basis for walnut germplasm resourced protection, development and application of hybridization breeding.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2014年第6期24-31,共8页
Journal of Northeast Agricultural University
基金
河南省重大科技攻关项目(092101110600)
山西农业大学创新基金项目(412500)
关键词
核桃
MATK序列
早实类群
进化树
walnut
matK sequence
early bearing walnut
evolutionary tree
