摘要
目的 探讨宫颈鳞癌组织中表皮生长因子（EGFR）的表达及其基因状态,并分析EGFR的表达与基因状态之间的相关性.方法 构建含有72例宫颈鳞癌组织标本的组织芯片,应用免疫组化方法和荧光原位杂交（FISH）技术检测EGFR蛋白的表达、EGFR基因扩增、基因拷贝数的变化.结果 72例宫颈鳞癌中,因2例组织点发生脱片、移位,1例定位有误（未见癌细胞）,剔除此3例,最终观察69例;EGFR蛋白表达的阳性率与肿瘤浸润深度、淋巴结转移及淋巴脉管间隙浸润有相关性（P均＜0.05）.FISH技术分析结果显示,72例宫颈鳞癌中有效信号共64例,基因扩增者7例,二倍体25例,三倍体23例,多倍体9例;所有EGFR基因扩增患者EGFR蛋白表达均为强阳性;EGFR蛋白表达与基因拷贝数增加之间有相关性（x2 =13.564,P＜0.05）.结论 EGFR蛋白过表达及基因状态可能参与宫颈鳞癌的生长、侵袭和转移过程,EGFR蛋白过表达可能由基因扩增或基因拷贝数增加所致.EGFR基因状态有可能成为宫颈鳞癌靶向治疗的有效监测指标.
Objective To elucidate the protein expression and gene expression status and the relationship between epidermal growth factor receptor （EGFR） protein expression and EGFR gene status.Methods Tissue microarray containing 72 cervical squamous cell carcinoma tissues was constructed,and EGFR protein expression and gene status were evaluated by immunohistochemical and fluorescence in situ hybridization （FISH） techniques.Results Protein expression of EGFR：69 of 72 cervical squamous cell carcinomas were observed.The results demonstrated it was significant association with invasion depth,lymphnode metastasis and lymph-vessel invasion （x2 =4.998,P ＜ 0.05 ; x2 =4.299,P ＜ 0.05 ; x2 =4.686,P ＜ 0.05） in cervical squamous cell carcinomas.For FISH assessing EGFR gene,64 of 72 carcinomas were observed; 7 of 64 cases showed EGFR gene amplification,and 25 disomy,23 trisomy and 9 polysomy were detected.There were high levels of protein expression in all the EGFR gene amplification cases,and there were significant association between EGFR protein expression and the gene copy number （x2 =13.564,P ＜ 0.05）.Conclusions EGFR may participate in the occurrence,progression and metastasis of cervical squamous cell carcinoma.Overexpression of EGFR protein may result from gene amplification and gene copy number increases,which showed that EGFR gene expression status may be a more effective biological indicator of cervical squamous cell carcinoma targeted therapy.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2013年第11期843-846,共4页
Chinese Journal of Obstetrics and Gynecology
基金
温州市科技计划（Y20100315）
关键词
宫颈肿瘤
癌
鳞状细胞
受体
表皮生长因子
基因扩增
原位杂交
荧光
免疫组织化学
Uterine cervical neoplasms
Carcinoma, squamous cell
Receptor, epidermal growth factor
Gene amplification
In situ hybridization, fluorescence
Immunohistochemistry
