摘要
背景:利用可降解组织工程骨材料治疗骨缺损是目前的最佳选择,如何保证材料充分地血管化已成为骨组织工程从基础研究向临床应用过渡的关键性问题。目的:综述可降解组织工程骨材料及促进其血管化的方法。方法:由第一作者检索PubMed数据库及PMC数据库1993至2013年有关可降解组织工程骨材料及促进其血管化方法的文献,检索词为"degradable materials;bone tissue engineering;angiogenesis;vasculogenesis;osteogenesis"。结果与结论:目前常用的可降解组织工程骨材料包括合成高分子材料、镁合金、生物活性玻璃、钙磷盐陶瓷及其复合物。可从支架构建,生长因子、内皮祖细胞的应用,细胞共培养、显微外科技术支持等方面促进组织工程骨材料血管化。虽然血管化组织工程骨研究取得了很大发展,但仍然存在诸多不足。相信随着血管形成机制及分子基础研究的深入、改进材料的结构性能、控释生长因子及提高其协同作用能力、优化种子细胞混合培养及提高显微外科技术,血管化组织工程骨将具有日益广阔的前景。
BACKGROUND：Using degradable materials for bone tissue engineering to treat bone defects is the best choice at present. How to ensure adequate vascularization in materials in order to meet the need of blood supply and an exchange of nutrients for seed cells in the process of bone regeneration is becoming a critical problem reversed from basic research to clinical application.
OBJECTIVE：To review the biodegradable materials for bone tissue engineering and the ways to promote vascularization in materials.
METHODS：A computer-based search of PubMed and PMC databases was performed by the first author for the literature about biodegradable materials for bone tissue engineering and their vascularization published from 1993 to 2013. The key words were“degradable materials, bone tissue engineering, angiogenesis, vasculogenesis, osteogenesis”in English.
RESULTS AND CONCLUSION：The commonly used biodegradable materials for bone tissue engineering include synthetic polymer materials, magnesium al oy, bioactive glass ceramics, calcium phosphate ceramics and composites. We can promote the vascularization of tissue-engineered bone by the fol owing aspects：scaffold fabrication, growth factors, the application of endothelial progenitor cells, cells co-culture and microsurgical techniques. Although the research on the vascularization of tissue-engineered bone has made a great progress, there is stil a lot of shortcomings. We believe that, with the development of mechanism of angiogenesis, the vascularized tissue-engineered bone wil have a promising prospect because of improving the structure and properties of materials, promoting the ability of release-control ed growth factors and their synergies, optimizing the selection and mixed culture of seed cells and improving the microsurgical techniques.
出处
《中国组织工程研究》
CAS
CSCD
2014年第21期3391-3397,共7页
Chinese Journal of Tissue Engineering Research
基金
重庆市科技攻关计划项目(CSTC2011ggB1004)
国家临床重点专科建设项目资助~~
