摘要
目的 :探讨吡柔比星联合雷公藤甲素对人膀胱癌T24细胞生长的影响及可能的机制。方法 :采用不同浓度的吡柔比星与雷公藤甲素单独或联合作用于T24细胞。应用MTT法检测对T24细胞生长抑制率的影响,FCM法检测细胞周期的变化,溴化去氧尿苷(bromodeoxyuridine,BrdU)渗入法分析单细胞DNA的合成情况,蛋白质印迹法检测对增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)蛋白表达的影响。结果 :吡柔比星与雷公藤甲素均可抑制T24细胞的生长(P<0.05),且呈剂量和时间依赖性。吡柔比星或吡柔比星联合雷公藤甲素均可增加G1和G2期细胞所占的比例,降低S期细胞所占的比例,双药联合可抑制单细胞DNA的合成。蛋白质印迹法检测结果显示,PCNA蛋白的表达水平明显下调,吡柔比星与雷公藤甲素联合处理具有协同效应。结论 :吡柔比星和雷公藤甲素均可抑制人膀胱癌T24细胞的生长,使细胞周期阻滞在G1期和G2期,两种药物对膀胱癌T24细胞的作用具有协同效应。
Objective： To investigate the growth inhibition effects of pirarubicin and triptolide on human bladder cancer T24 cells, and to explore the possible mechanism. Methods： The T24 cells were treated with different concentrations of pirarubicin or triptolide alone or in combination. The growth inhibition rate of T24 cells was detected by MTT method, and the change of cell cycle distribution was analyzed by flow cytometry （FCM）. The DNA synthesis of individual cell was analyzed by bromodeoxyuridine （BrdU） incorporation. The expression level of proliferating cell nuclear antigen （PCNA） protein in T24 cells was examined by Western blotting. Results： The growth of T24 cells was inhibited by pirarubicin or triptolide in a dose- and time-dependent manner （P 〈 0.05）. The percentage of cells in G1-G2 phase was increased while which in S phase was decreased by the treatment of pirarubicin or pirarubicin in combination with triptolide. The DNA synthesis of individual cell was inhibited by pirarubicin in combination with triptolide. The expression level of PCNA was signifcantly decreased, and the combination regimen of pirarubicin and triptolide exerted a synergistic effect. Conclusion： Pirarubicin and triptolide can both inhibit the proliferation of human bladder cancer T24 cells and arrest the cells at G1-G2 phase. The combination regimen of pirarubicin and triptolide exerts a synergistic effect.
出处
《肿瘤》
CAS
CSCD
北大核心
2014年第6期514-519,共6页
Tumor
基金
丹阳市社会发展科技专项基金(编号:DK201214)
关键词
膀胱肿瘤
抗肿瘤联合方案
吡柔比星
雷公藤甲素
T24细胞
Urinary bladder neoplasms
Antineoplastic combined chemotherapy protocols
Pirarubicin
Triptolide
T24 cells
