摘要
建立采用UHPLC同时测定野菊花中绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸、4,5-二咖啡酰奎尼酸和蒙花苷含量的分析方法。UHPLC分析条件：Agilent ZORBAX RH C18色谱柱（50 mm×2.1 mm,1.8μm）,检测波长326 nm,流动相为乙腈-0.1%磷酸水进行梯度洗脱,流速0.4 mL/min,柱温25℃。实验结果显示绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸、4,5-二咖啡酰奎尼酸、蒙花苷分别在1.20-24.00、1.16-23.20、2.23~44.60、1.75-35.00、2.25-45.00μg/mL范围内线性关系良好（r〉0.9998）,平均回收率分别为98.08%、98.42%、97.75%、98.27%、98.36%;RSD分别为0.38%、1.50%、0.77%、0.81%、0.62%。UHPLC法分析速度快、高效、重复性好、结果准确简便,可用于野菊花中四种有机酸和蒙花苷含量的测定。
The objective of this study was to develop a UHPLC method for the simultaneous determination of five active components, namely chlorogenic acid, 3,4-O-dlcaffeoylquinic acid, 3,5-odicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid and huddieoside in Flos Chrysanthemi Indlci. The chromatographic separation was performed on a Agilent ZORBAX RH C18 （50 mm×2.1 mm,1.8μm） column with acetonitrile-0.1% phosphoric buffer as mobile phases. The flow rate was 0.4 mL/min. The DAD detection wavelength was set at 326 nm and the column oven temperature was maintained at 25 ℃. Chlorogertie acid,3,4-O-dieaffeoylquinic acld,3,5-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid and bud- dleaside showed good linearity （ r 〉 0.9998 ） within the range of 1.20-24.00,1.16-23.20,2.23-44.60,1.75-35.00,2. 25-45.00 μg/mL,respectively; their respective average recoveries were 98.08% （ RSD = 0. 38% ） ,98.42% （ RSD = 1. 50% ） ,97.75% （ RSD = 0.77% ） ,98.27% （ RSD = 0.81% ） ,98.36% （ RSD = 0.62% ）. Based on these results, it was concIudod that the developed UHPLC method was simple, sensitive, accurate and reproducible. It can be used for the quality control of the five active components of Flos Chrysanthemi Indici.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第6期890-894,共5页
Natural Product Research and Development
基金
河南省教育厅自然科学研究计划项目(2010A360016)
