摘要
目的:建立岗梅感冒颗粒的特征图谱,并同时测定(R,S)-告依春、新绿原酸、绿原酸、隐绿原酸、甘草苷、吴茱萸春碱和甘草酸7个成分的含量。方法:采用超高效液相(UPLC)色谱法,色谱柱为Waters BEH C18(150mm×2.1mm,1.7μm),以乙腈-0.1%磷酸为流动相进行梯度洗脱,检测波长为241nm,柱温为40℃,流速为0.3mL/min;分别对10批岗梅感冒颗粒的特征图谱进行质量评价及含量测定。结果:建立了岗梅感冒颗粒的UPLC特征图谱,共标定了15个共有峰,利用《中药色谱指纹图谱相似度评价系统(2012,130723版本)》软件对10批样品指纹图谱进行分析,各批次样品相似度均在0.9以上。所建立的色谱条件下的7个成分(R,S)-告依春、新绿原酸、绿原酸、隐绿原酸、甘草苷、吴茱萸春碱和甘草酸分离度良好,各成分进样量分别在0.907~29.029ng、3.83~122.50ng、5.76~184.31ng、5.67~181.30ng、6.99~223.72ng、0.123~3.932ng、17.69~565.98ng范围内与峰面积呈良好的线性关系,平均回收率分别为101.4%、97.4%、99.8%、97.2%、98.9%、93.6%、97.5%,RSD分别为1.9%、1.5%、0.8%、1.5%、1.2%、1.0%、1.7%(n=6)。结论:该研究建立的UPLC特征图谱及多指标成分含量测定分析方法灵敏度高、专属性强,可用于岗梅感冒颗粒的质量控制。
Objective: To establish an UPLC fingerprint of Gangmei Ganmaoling Granules, and determine the content of(R,S)-goitrin, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, evolitrine and glycyrrhizic acid. Methods: UPLC method was adopted with the Waters BEH C18 column(150 mm × 2.1 mm, 1.7μm), the mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution, the detection wavelength was 241 nm, the colunm temperature was 40℃, the flow rate was 0.3 mL/min and the sample size was 1μL. The content determination and quality evaluation of characteristic fingerprints of 10 batches of Gangmei Ganmao Granules were carried out. Results: The UPLC characteristic fingerprints of Gangmei Ganmao Granules was established, the common mode of fingerprint was set up with 15 common peaks, the fingerprint of 10 batches of samples were analyzed by using "Chinese Medicine Fingerprint Similarity Evaluation System(2012, 130723 versions)", the similar degrees of 10 batches of samples were over 0.9. Under the established chromatographic conditions, 7 indicative components of Gangmei Ganmao Granules,and determine the content of(R,S)-Goitrin, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, evolitrine and glycyrrhizic acid were well separated, each component has a good linear relationship separately in the range of 0.907~29.029 ng, 3.83~122.50 ng, 5.76~184.31 ng,5.67~181.30 ng, 6.99~223.72 ng, 0.123~3.932 ng, 17.69~565.98 ng, the average recoveries were 101.4%, 97.4%, 99.8%, 97.2%, 98.9%, 93.6%, 97.5%,RSDs were 1.9%, 1.5%, 0.8%, 1.5%, 1.2%, 1.0%, 1.7%(n=6). Conclusion: The estabilshed method for UPLC characteristic fingerprint and content determination of muti index components of Gangmei Ganmao Granules is sensitive and specific, which can be used for quality control of Gangmei Ganmao Granules.
作者
黄际薇
吴晓纯
罗文安
何广铭
HUANG Ji-wei;WU Xiao-chun;LUO Wen-an;HE Guang-ming(The Third Affiliated Hospital,Sun Yat-Sen University,Guangzhou,Guangdong510630;Guangdong Yifang Pharmaceutical Co. Ltd,Foshan,Guangdong 528244)
出处
《按摩与康复医学》
2019年第15期40-44,共5页
Chinese Manipulation and Rehabilitation Medicine
基金
广州市科技攻关专项资助项目,编号:2012J4300071
关键词
岗梅感冒颗粒
UPLC
特征图谱
吴茱萸春碱
含量测定
Gangmei Ganmao Granules
UPLC
characteristic fingerprint
evolitrine
content determination
