摘要
为进一步发展新型Robson大环配合物体系,在三价铒离子的存在下,用2,6-二甲酰基-4-甲基苯酚和三(3-氨基丙基)胺(trpn)通过[2+2]模板缩合反应直接合成了一个新型的双臂大环单核稀土铒配合物,通过物理方法对其进行了结构表征,用凝胶电泳实验和抗菌实验初步考察了配合物的生物活性.电喷雾质谱研究表明:配合物为[2+2]缩合反应生成的单核结构.凝胶电泳实验研究表明:标题配合物能将DNA切割成环形缺刻型DNA,并以插入方式与相DNA结合;同时抑菌毒性实验证实了配合物对大肠杆菌也有一定的抑制作用.
To develop new Robson macrocyclic complexes,a new suspended-armed macrocyclic erbium complex was synthesized by [2＋2] condensation of 2,6-diformyl-4-methyl phenol and three （3-aminopropyl） amine （trpn） in the presence of erbium ion.The title complex was characterized by physical methods,and the biological activities of the complex were studied by agarose gel electrophoresis and antibacterial toxic experiment.The electrospray mass spectrum of the complex shows that the complex is a mononuclear complex which is derived from [2＋ 2] condensation reaction.The study of agarose gel electrophores shows that the complex can cleave the pBR322 DNA into an open circular relaxed form,and the complex binds to DNA mainly in an intercalating mode.The results of antibacterial toxic experiment show that the title complexes have inhibiting effects on escherichia coli.
出处
《武汉工程大学学报》
CAS
2014年第4期12-16,共5页
Journal of Wuhan Institute of Technology
基金
湖北省自然科学基金重点项目(2011CDA048)
关键词
稀土
大环配体
切割DNA
抑菌
rare earth
macrocyclic complex
DNA cleavage
inhibition of escherichia coli
