摘要
目的探讨抗氧化剂褪黑素(MEL)对间歇低氧(IH)小鼠胰岛素抵抗的干预作用及其可能机制。方法雄性C57BL/6J小鼠45只随机分成常氧对照组(NC组)、低氧对照组(CIH组)及低氧干预组(CIH+M组),每组各15只。成功复制动物模型后,测定各组小鼠胰岛素抵抗水平、胰腺组织中各种氧化应激指标。结果与NC组比较,CIH组胰岛素抵抗水平(30、60和90 min时间点)及胰腺组织中氧化应激水平显著增加(P<0.01);而CIH+M组上述各种指标较CIH组显著降低(P<0.01);CIH+M组与NC组比较上述各种指标无明显差异(P>0.05)。结论抗氧化剂MEL可通过其抗氧化作用干预IH所致胰岛素抵抗的发生。
[Objective] To explore the effect and mechanism of antioxidants melatonin （MEL） against in-termittent hypoxia （IH）-induced insulin resistance in mice. [Methods] Forty-five male C57BL/6J mouse wererandomly assigned to 3 groups： normoxic control group （NC group）, conventional intermittent hypoxia group（CIH group）, intermittent hypoxia MEL treatment group （CIH＋M group）, with 15 mouse in each group. Afterthe experiment, insulin resistance levels, various indexes of oxidative stress in pancreatic tissue were measuredin all groups. [Results] Compared with the NC group, the insulin resistance levels （30, 60 and 90 rain） andindexes of oxidative stress in pancreatic tissue were increased （all P〈0.01）; But all above indexes were sig-nificantly lower in CIH＋M group compared with those in CIH group （P 〈0.01）; there were not significantlydifferent between CIH＋M group and NC group in all above indexes （P〉0.05）. [Conclusion] Antioxidant MELcould prevent CIH-induced insulin resistance by its anti-oxidative effect.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2014年第14期28-31,共4页
China Journal of Modern Medicine
基金
辽宁省葫芦岛市科技局社会发展类课题(No:12702)
关键词
抗氧化剂
间歇低氧
氧化应激
胰岛素抵抗
antioxidants
intermittent hypoxia
oxidative stress
insulin resistance
