摘要
目的探讨葡萄籽原花青素对睡眠呼吸暂停模式低氧大鼠海马区磷酸化丝裂原活化蛋白激酶(p38MAPK)和白介素-1β(IL-1β)表达的影响。方法 80只雄性SD大鼠随机分成对照组、模型组及高、低剂量萄籽原花青素组。对照组暴露于空气中,模型组暴露于低氧条件下(50ml/L,暴露时间每天8h,持续时间2、6w)。电镜观察海马区神经细胞超微结构,免疫印迹检测磷酸化p38 MAPK和IL-1β的表达变化,水迷宫测试动物学习记忆功能。结果与对照组比较,模型组神经细胞结构损伤、磷酸化p38 MAPK IL-1β的表达升高,水迷宫检测动物逃避潜伏期时间延长、穿台次数减少(P<0.05);与模型组比较,葡萄籽原花青素能减轻神经细胞超微结构的损伤,降低磷酸化p38 MAPK和IL-1β表达,水迷宫检测动物逃避潜伏期时间缩短、穿台次数增多(P<0.05);上述变化在高剂量萄籽原花青素组最为显著(P<0.05)。结论葡萄籽原花青素能改善睡眠呼吸暂停模式低氧大鼠的学习记忆功能,与抑制海马区磷酸化p38 MAPK和IL-1β表达有关。
Objective To investigate the effects of Grape seed proanthocyanidin on expression of phosphorylated p38MAPK and IL-1β in hippocampus in a rat model of obstructive sleep apnea hypoxia. Method We randomly divided 80 Male SD rats into four groups ： control group, model group, low dose of Grape seed proanthoeyanidin treatment group and high dose of Grape seed proanthocyanidin treatment group. Rats in control group were exposed in air, the model group were suf- fered from intermittent hypoxia conditions （5Oral/L, 8-hour--intermittent hypoxia everyday, and the duration of experiment was 2 and 6 weeks, respectively）. Electron microscope was used to observed neuron pathology in hippocampal region;West- ern-Blotting was used to observed the expressions of phosphorylated p38MAPK and interleukin1β proteins. Morris water maze （MWM）was used to assess memory-learning function. Results Compared with the control group, neuronal pathology injury was significantly injured;The expressions of phosphorylated p38MAPK and IL-1β proteins significantly increased（ P 〈 0.05 ） ; MWM test showed that the escaping latency prolonged and the frequency of crossing the platform decreased. Com- pared with model group ,phosphorylated p38MAPK and IL-1β level decreased in Grape seed proanthocyanidin（ P 〈 0.05 ） and the escaping latency shorted and the frequency of crossing the platform increased;What the above mentioned changes were more significant in high dose of Grape seed proanthocyanidin group （P 〈 0.05）. Conclusion Grape seed proanthocy- anidin could improve learning memory function after obstructive sleep apnea hypoxia, which is related to inhibiting phospho- rylated p38MAPK and IL-1β in hippocampal region.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2013年第11期989-992,共4页
Journal of Apoplexy and Nervous Diseases
基金
河北省教育厅重点资助项目(No.ZH2012046)
