摘要
目的探讨抗氧化剂氮氧化物4-羟基-2,2,6,6-四甲基哌啶(TEMPOL)和还原型谷胱甘肽(GSH)对间歇低氧(IH)小鼠胰腺损伤的保护作用。方法雄性C57BL/6J小鼠50只按随机数字表法随机均分为正常对照(CON)组、IH组、IH+TEMPOL组、IH+GSH组和IH+TEMPOL+GSH组。成功制备动物模型后,测定各组小鼠胰岛素抵抗(IR)水平,胰腺组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)的含量及胰腺β细胞的凋亡率。结果IH组血糖水平高于CON组,IH+TEMPOL+GSH组血糖水平低于IH组(P<0.01)。IH组胰腺组织中MDA(μmol/g蛋白)水平高于CON组(4.48±0.25 vs 1.94±0.21),IH组的SOD(U/mg蛋白)和GR(mg/g蛋白)水平低于CON组(61.52±3.33vs 100.05±7.26,107.81±7.54 vs 170.54±4.90,P<0.01);IH+TEMPOL+GSH组MDA水平(2.07±0.35)低于IH组,IH+TEMPOL+GSH组SOD及GR水平(96.68±5.85和166.87±5.75)高于IH组(P<0.01)。IH组较胰腺β细胞凋亡率(%)高于CON组(2.43±0.07 vs 0.54±0.06),IH+TEMPOL+GSH组凋亡率(0.56±0.06)低于IH组(P<0.01)。IH组、IH+TEMPOL组和IH+GSH组与间上述各种指标差异均无统计学意义(均P>0.05)。结论抗氧化剂TEMPOL联合GSH对IH所致小鼠胰腺损伤具有明显的保护作用,而单独应用抗氧化剂TEMPOL或GSH对IH所致胰腺损伤无明显的保护作用。
Objective To explore the protective effect of co-treatment with antioxidants 4-hydroxy-2,2,6,6-tetra-methylpiperidine 1-oxyl （TEMPOL） and reduced glutathione （GSH） in pancreatic injury induced by intermittent hypoxia （IH） in mice. Methods Fifty male C57BL/6J mice were randomly divided into control group（CON）, IH group （IH）, IH＋TEM-POL group （IH＋TEMPOL）, IH＋GSH group （IH＋GSH） and IH＋TEMPOL＋GSH group （IH＋TEMPOL＋GSH）. After successful-ly prepared animal model, the insulin resistance （IR） level, pancreatic malondialdehyde （MDA） level,superoxide dismutase （SOD） activity,glutathione reductase （GR） concentration and pancreatic β-cell apoptosis were detected in five groups. Re-sults The blood glucose level was significantly increased in IH group than that in CON group （P〈0.01）, but which was sig-nificantly decreased in IH＋TEMPOL＋GSH group than that of IH group （P〈0.01）. The MDA content was significantly higher in the IH group than that in CON group （4.48±0.25 vs 1.94±0.21, P〈0.01）, but SOD activity and GR content were signifi-cantly lower in IH group than those of CON group （61.52±3.33 vs 100.05± 7.26,107.81±7.54 vs 170.54±4.90,P〈0.01）. The level of MDA was significantly lower in IH＋TEMPOL＋GSH group （2.07±0.35） than that in IH group. The levels of SOD and GR were significantly higher in IH＋TEMPOL＋GSH group （96.68±5.85 and 166.87±5.75） than those of IH group （P〈0.01）. The apoptotic rate of pancreaticβcells was significantly increased in IH group than that in CON group （2.43±0.07 vs 0.54± 0.06, P〈0.01）, but it was significantly lower in IH＋TEMPOL＋GSH group （0.56 ± 0.06） than that in IH group （P〈0.01）. There were no significantly differences in all above indexes between IH group, IH＋TEMPOL group and IH＋GSH group （ P＆gt;0.05）. Conclusion The co-treatment with the antioxidant TEMPOL and GSH can obviously protect IH-induced pancreatic injury in mice. However, there was no
出处
《天津医药》
CAS
北大核心
2014年第2期170-172,I0004,共4页
Tianjin Medical Journal
基金
辽宁省葫芦岛市科技局社会发展类资助项目(项目编号:12702)
