摘要
目的探讨CD4+CD25+Foxp3+/CD4+CD25+检测与急性髓系白血病的发病机制、治疗效果及预后的关系,并与CD4+CD25+/CD4+法比较两种检测方法是否存在差异。方法应用流式细胞术法分别检测41例初治AML患者(初治组)、11例一次化疗缓解的患者(化疗敏感组)、9例两次以上化疗不缓解的患者(化疗耐药组)、13例化疗缓解后一直没有复发的患者(持续缓解组)、8例化疗缓解后又再次复发的患者(复发组)和20例健康体检者(对照组)CD4+CD25+Foxp3+/CD4+CD25+及CD4+CD25+/CD4+在外周血中的比例。结果与对照组相比较,AML患者初治组两类细胞比例均较正常人明显升高(P<0.01),初治患者组两种检测方法间存在显著差异(P<0.01)。与化疗缓解组比较,化疗耐药组两种检测方法检测的比例均明显升高(P<0.01),耐药患者组两种方法间存在显著差异(P<0.01)。与持续缓解组比较,复发组患者的比例明显升高(P<0.01),复发组两种方法间存在显著差异(P<0.01)。结论急性髓系白血病患者外周血中CD4+CD25+Foxp3+/CD4+CD25+与急性髓系白血病的发生相关,并且检测其比例能预测白血病患者对化疗药物的反应及预后,且其灵敏度高于CD4+CD25+/CD4+检测法,而其特异性与CD4+Foxp3+/CD4+检测法一致。
Objective To explore the significance of CD4＋CD25＋Foxp3＋Tregs detection in the pathogenesis, curative effect and prognosis of acute leukemia, and compare it with the method of CD4＋CD25＋/CD4＋detection and determine whether there is differ-ence between the two methods. Methods The proportion of CD4＋CD25＋Foxp3＋Tregs cells in the peripheral blood were tested in 41 cases of untreated patients with AML （Initial treatment group）, 11 cases of remission-patients after one period of chemotherapy （Chemotherapy sensitive group）, 9 cases of non-remission-patients after two period of chemotherapy （Chemotherapy resistance group）, 13 cases of recurrence-free-patients achieving chemotherapy remission （Sustained remission group）,8 relapsed patients achieving Chemotherapy remission （Relapse group）, 20 cases of healthy donators （control group） by using flow cytometry, and the proportion of CD4＋CD25＋Foxp＋Tregs cells in the peripheral blood with the method of CD4＋CD25＋Foxp3＋/CD4＋CD25＋and CD4＋CD25＋/CD4＋were detected. Results The proportion of CD4＋CD25＋Foxp3＋Tregs cells in the peripheral blood of initial treatment group was greatly higher than that of control group, but there was no difference between the two methods of CD4＋CD25＋Foxp3＋/ CD4＋CD25＋and CD4＋CD25＋/CD4＋ detection. The proportion of CD4＋CD25＋Foxp3＋ Tregs cells in the peripheral blood of chemotherapy resis-tance group was greatly higher than that of chemotherapy sensitive group, but there was no difference between the two methods of CD4＋CD25＋Foxp3＋/CD4＋CD25＋and CD4＋CD25＋/CD4＋detection in the chemotherapy resistance group and chemotherapy sensitive group. The proportion of CD4＋CD25＋Foxp3＋Tregs cells in the peripheral blood of relapse group was greatly higher than that of sus-tained remission group, but there was no difference between the two methods of CD4＋CD25＋Foxp3＋/CD4＋CD25＋and CD4＋CD25＋/CD4＋ detection in the relapse group and sustained remission g
出处
《实验与检验医学》
CAS
2014年第3期246-248,251,共4页
Experimental and Laboratory Medicine
基金
江西省卫生厅科技计划(编号:20093049)
