摘要
目的:观察原发性肝癌(HCC)患者外周血和肝脏CD4+CD25+FoxP3+调节性T细胞(Treg)的频率、表型和分布特点。方法:应用流式细胞术分析30例原发性肝癌患者、30例健康人的外周血或HCC组织中肿瘤浸润淋巴细胞(TIL)和非肿瘤浸润淋巴细胞(NIL),Treg细胞的特点,并分析其临床意义。结果:结果表明HCC患者外周血Treg细胞(7.49±2.87)%明显高于健康对照组(4.36%±1.61%,P<0.01);肝癌组织中Treg细胞频率(23.48%±27.82%)明显高于癌旁组织(6.19%±3.10%,TILvsNIL,P<0.05)。此外,我们观察到TIL中CD4+CD25low和CD4+CD25-细胞亚群的表达FoxP3蛋白的水平显著升高。结论:在HCC患者外周血及肝脏肿瘤区Treg细胞频率均明显升高,可能与体内Treg来源增加有关,而增加的Treg通过进一步抑制宿主免疫应答而加快肝癌的临床进展。
AIM： This study attempted to investigate the features of Treg cells in peripheral blood and liver of patients with primary hepatocellular carcinoma（HCC）.METHODS： Peripheral blood samples were obtained from 30 HCC patients and 30 healthy control subjects,then were quantitatively analyzed for Treg cells by using flow cytometry.Liver infiltrating lymphocytes（LIL） isolated from resected tumor samples of 7 HCC patients were simultaneously analyzed.RESULTS： There was a significant increase in the frequency of peripheral Treg cells in HCC patients compared with healthy controls（P〈0.01）.Furthermore,we also found that there was a higher frequency of infiltrated Treg within tumor samples than the counterpart in non-tumor region（P〈0.05）.In addition,CD4＋ CD25low and CD4＋ CD25-T cells isolated from resected tumor samples were found to express higher level of FOXP3 molecules.CONCLUSION： Our findings showed that a dramatic increasing increase of Treg in peripheral blood and liver tissue of HCC patients may be associated with the significant increased development of Treg,which favors the disease progression through the suppressive effect of Treg on host immune response in these patients.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第6期668-670,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30901328)
国家重点基础研究发展计划(973)资助项目(2009CB522500)
关键词
原发性肝癌
T细胞
致病机制
primary carcinoma of liver
T cell
pathogenic mechanism
