摘要
目的:观察补肾益气方对自然衰老大鼠皮质酮及IL-2基因表达的影响。方法:将20月龄老年大鼠随机分为对照组与补肾组,各10只。另设3月龄青年组。其中老年补肾组自分组日起以饮料方式饲喂补肾益气方药液,连续3个月,其余两组予正常饮水和饲料。于干预3月结束时,取腹主动脉血和脾脏。采用ELISA法检测大鼠血清IL-2含量;EIA法检测大鼠血清皮质酮含量;qRT-PCR法检测大鼠脾淋巴细胞中IL-2、糖皮质激素受体(GR)mRNA的表达;Western blotting法检测大鼠脾淋巴细胞GR蛋白表达。结果:与青年组相比,老年对照组大鼠血清皮质酮含量明显升高(P<0.05),血清IL-2含量明显降低(P<0.05),脾淋巴细胞IL-2 mRNA、GR mRNA和蛋白表达明显下降(P<0.05);与老年对照组比较,老年补肾组血清IL-2水平明显升高(P<0.05),GR mRNA和蛋白表达明显升高(P<0.05)。结论:补肾益气方可通过调节老年大鼠异常的皮质酮/糖皮质激素受体通路,改善IL-2基因表达的增龄性衰退。
Objective： To investigate the effect of ＂ Bushen Yiqi Formula＂ on expression of IL-2 and secretion of endogenous corticosterone in senescent rats. Methods： Twenty months rats were randomized into control group and kidney- replenishing group, with 10 rats ineach group Young group was established, Kidney-replenishing group was treated with ＂Bushen Yiqi Formula＂ for 3 months continuously, and the others were treated with water and fodder. The content of serum IL-2 was detected by ELISA and the corticosterone content was detected by EIA; the mRNA expressions of IL-2 and GR in splenic lymphocytes were detected by qRT-PCR; the expression of glucocorticoid receptors （GR） protein was detected by Western blotting. Results： Compared with young group, the corticosterone content in aged control group was increased markedly （P 〈 0.05 ）, but the IL-2 mRNA expression in splenic lymphocytes, serum IL-2 and GR expression were decreased markedly （ P 〈 0.05 ） ; Compared with aged control group, the expressions of IL- 2 and GR were improved in aged kidney- replenishing group, and the content of corticosterone was down-regulated. Conclusion： ＂Bushen Yiqi Formula＂ can improve the IL-2 expression by regulating corticosterone/GR pathway in aged rats.
出处
《上海中医药大学学报》
CAS
2014年第3期54-56,67,共4页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81001565)
上海市教委"085"工程学科建设科技创新支撑计划(085ZY1204)
上海市教委重点学科建设项目(J50301)
国家基础科学人才培养基金项目(J1103607)
关键词
衰老
补肾益气方
皮质酮
糖皮质激素受体
白介素2
大鼠
senescence
＂Bushen Yiqi Formula＂
corticosterone
glucocorticoid receptors （GR）
IL-2
rat
