摘要
以海藻酸钠-琼脂为载体,对啤酒酵母细胞进行固定化。通过正交试验确定了细胞固定化的最佳制备条件,载体浓度为1.4 g/40 mL,固化时间为2 h,复合比例为1∶1,固化温度为46℃,固定化效果最好。在最佳载体固定化条件下,对固定化酵母发酵工艺条件进行初步研究,最优条件为:接种温度13℃,填充率60%,pH5,流量6 L/h。
Beer yeast cell was immobilized by sodium alginate and agar as the carrier. The optimum immobilization conditions were determined as follows through orthogonal experiments： the concentration of the carrier was 1.4 g/40 mL, the immobilizing time was 2 h, the ratio of composite carrier was 1：1, and the immobilizing temperature was at 46 ℃. Then the fermenting conditions of the immobilized yeast were studied and the best conditions were summed up as follows： the inoculating temperature was at 13 ℃, the filling rate was 60 %, pH5, and the flowing rate was 6L/h.
出处
《酿酒科技》
北大核心
2014年第6期71-73,共3页
Liquor-Making Science & Technology
关键词
微生物
固定化酵母
优化
后发酵
啤酒
microbe
immobilized yeast
optimization
continuous fermentation
beer
