摘要
目的观察慢性阻塞性肺疾病(COPD)大鼠调节性T细胞(Treg)、叉头状转录因子3(Foxp3)、T-bet、GATA连接蛋白3(GATA3)的变化。方法将30只大鼠随机分为对照组、模型组,每组15只。模型组大鼠采用烟熏加脂多糖(LPS)气管滴入方法建立COPD模型。模型复制成功第28天,采用ELISA检测大鼠血清、支气管肺泡灌洗液(BALF)白介素4(IL-4)、γ干扰素(IFN-γ)水平,流式细胞术检测外周血Treg表达,分别采用逆转录PCR及Western blot法检测肺组织Foxp3、T-bet、GATA3mRNA和蛋白表达。结果模型组大鼠肺泡腔及肺间质内大量炎性细胞浸润,肺组织结构破坏。与对照组比较,模型组大鼠血清IFN-γ表达升高(P<0.01),IL-4、CD4+CD25+Treg、CD4+CD25+Foxp3+Treg降低(P<0.05);模型组Foxp3、GATA-3mRNA、蛋白表达降低,T-bet mRNA和蛋白升高(P<0.01)。相关性分析显示,T-bet、GATA-3、Foxp3基因和蛋白表达与IL-4、IFN-γ分泌相关(P<0.05)。结论 COPD炎症反应与T-bet、GATA-3、Foxp3表达失衡有一定关系。
Objective To observe the change of regulatory T cells（Tregs）, fork head-like transcription factor 3 （ Foxp3）, T box expressed in T cells （T-bet） and GATA binding protein 3 （GATA3） in rat models of chronic obstructive pulmonary disease （COPD）. Methods Thirty rats were randomly divided into control group and model group （n = 15 each）. The rats of model group were developed by lipopolysaccharide （LPS） and smoking. Interleukin-4 （IL-4） and γ-interferon （IFN-y） in serum and bronchoalveolar lavage fluid （BALF） were detected 28 days after modeling by ELISA. Peripheral Tregs were detected by flow cytometry. The expressions of Foxp3, T-bet, GATA gene and protein in lung tissue were observed by reverse transcription PCR and Western blotting, respectively. Results Compared with the control group, the model group had more serious inflammation in lung tissues, expressed the higher levels of IFN-γ in serum and BALF （P 〈0.01 ）, T-bet mRNA and protein in lung tissue, and the lower levels of IL-4, CD4＋ CD25 ＋ Treg, CD4＋ CD25＋ Foxp3- Treg （ P〈0.05）, Foxp3, GATA-3 mRNA and protein （ P 〈 0.01 ）. Correlation analysis showed that there were correlations between T-bet, GATA-3, Foxp3 expressions and IL-4, IFN-γ levels （P 〈 0.05）. Conclusion There is a relationship between inflammation and imbalance of in the expression of T-bet, GATA-3, Foxp3 in COPD.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2014年第7期704-707,712,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81072781)
关键词
慢性阻塞性肺疾病
调节性T细胞
叉头状转录因子3
T-BET
GATA连接蛋白3
chronic obstructive pulmonary disease
regulatory T cells
fork head-like transcription factor 3
T box expressed in T cells
GATA binding protein 3
