摘要
试验旨在研究会理黑山羊线粒体DNA（mtDNA）D-loop区的起源进化及遗传多样性，为保护该品种山羊遗传资源提供理论依据。采用PCR扩增、测序、生物信息学分析等技术分析41只会理黑山羊mtDNAD-loop区核苷酸序列，并对其进行系统发育树构建。会理黑山羊mtDNAD-loop区的长度在1211～1213bp之间，其中2个个体为1211bp，29个个体为1212bp，10个个体为1213bp；A＋T（60．1％）含量远高于G＋C（39．9％）含量，有9种单倍型，单倍型多样度为0．842±0．00368，核苷酸多样度为0．01542±0．00034。遗传进化分析表明，会理黑山羊与建昌黑山羊、成都麻羊、金堂黑山羊、贵州白山羊、贵州黑山羊亲缘关系最近，与旋角野山羊亲缘关系最远。会理黑山羊具有较丰富的遗传多样性，在进化过程中受到其他品种山羊影响。
To explore the genetic diversity and origin for genetic resource protection of Huili Black goat, the mitochondrial DNA （mtDNA） D-loop was investigated, mtDNA D-loop sequences of 41 goats were analyzed by PCR, sequencing techniques, and biological information and the phylogenetic trees were constructed. The mtDNA sequences of the Huili Black goat ranged from 1211 to 1213 bp, and 2 sequences were 1211 bp, 29 sequences 1212 bp, and 10 sequences 1213 bp. The content of A＋T （60.1%） was higher than one of G＋C （39.9%）. There were 9 haplotypes, and the haplotype diversity was 0. 842＋0. 00368. The nucleotide diversity was 0. 01542＋0. 00034. The phylogenetic analysis showed that Huili Black goat was distributed in a branch, and were closed to Jianchang Black goat, Chengdu Ma goat, Jintang Black goat, Guizhou White goat, Guizhou Black goat, but they were less related to Capra falconeri. Huili Black goats had rather abundant genetic diversity, and were greatly affected by other goat breeds in history.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第6期172-176,共5页
China Animal Husbandry & Veterinary Medicine
基金
四川省教育厅重点项目(09ZA105)
四川省教育厅青年基金项目(09ZB075)
会理县畜牧局校县合作项目(XA0807)
