摘要
以离心换液的批培养为例,通过设计谷氨酰胺和天冬酰胺不同的添加方式来考察两者对CHO细胞生长,代谢及产物表达的影响。结果表明:基础培养基中谷氨酰胺和天冬酰胺不能简单地相互替换,缺失谷氨酰胺或天冬酰胺的基础培养基均不能支持dhfr-CHO细胞的正常生长和产物表达,仅谷氨酰胺和天冬酰胺的浓度同时达到4mmol/L,才能满足细胞生长所需。另外,代谢副产物氨的生成仅与谷氨酰胺和天冬酰胺的加和线性相关,与两者添加比例无关。但适当提高天冬酰胺与谷氨酰胺的比例可提高抗体表达水平,同时减少乳酸的生成。因此,为培养基开发与优化过程中谷氨酰胺和天冬酰胺的添加策略提供了依据,为建立高效的流加培养过程奠定了基础。
The effects of glutamine and asparagine on CHO cell growth, metabolism and antibody expression in batch culture with cells being centrifuged were investigated. It was found that glutamine can＇ t be replaced by asparagine simply, CHO-dhfr cells can＇t grow normally in adding separately experiments. When glutamine and asparagine concentration reached 4mmol/L meanwhile can support normal growth of CHO ceils. As the total amount of glutamine and asparagine increased （0 to 24mmol/L）, ammonia concentration increased linearly, however, ammonia production had no relationship with proportion of glutamine and asparagine. Furthermore, improving the asparagine and glutamine proportion moderately not only can increase the antibody production but also decrease the lactate production. In brief, the foundation of supplement of glutamine and asparagine in development and optimization of mediums and efficient fed-batch process for antibody production was established.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2014年第4期9-15,共7页
China Biotechnology
基金
国家"863"计划(2012AA02A303)资助项目
关键词
谷氨酰胺
天冬酰胺
比例
总量
抗体
氨
乳酸
Glutamine Asparagine Proportion Total amount Antibody Ammonia Lactate
