摘要
目的 探讨组织工程化肌腱对 T淋巴细胞亚群及受体的影响 ,明确其组织相容性。方法 采用3月龄法国罗曼鸡 75只 ,随机分成五组 ,每组 15只。手术造成右足第 3趾屈趾深肌腱 2 .5 cm缺损后 ,分组。A组 :未手术组 ;B组 :自体肌腱移植组 ;C组 :新鲜同种异体肌腱移植组 ;D组 :梯度降解材料复合腱细胞移植组 ;E组 :衍生肌腱材料复合腱细胞移植组。检测 CD4+ 、CD8+ 、CD2 8细胞阳性率及 T淋巴细胞相关抗原受体 (TCR)密度变化。通过刀豆蛋白 (Con A)诱导的淋巴细胞转化试验、直接淋巴细胞混合培养试验及间接淋巴细胞混合培养试验 ,了解机体对肌腱细胞的免疫反应水平。结果 D、E组与 B组比较 ,早期 CD4+ 、CD8+ 及 TCR轻度增高 ,2周后下降 ,无显著性差异 (P>0 .0 5 )。C组 CD4+ 、CD8+ 、CD2 8及 TCR阳性率与 D、E组比较 ,有显著性差异 (P<0 .0 5 )。结论 肌腱细胞为弱抗原细胞 ,抗原脱落或可溶性抗原分泌较少。
Objective To investigate the influence of tissue engineered tendon on subgroup of T lymphocytes and its receptor in Roman chickens. Methods The flexor digitorum profundus of the third toes of right feet in 75 Roman chickens were resected and made 2.5 cm defects as experimental model. They were randomly divided into five groups according to five repair methods: no operation (group A), autograft (group B), fresh allograft (group C), polymer combined with allogenous tendon cells (group D), derived tendon materials combined with allogenous tendon cells (group E). The proliferation and transformation of lymphocytes and contribution of CD4 +、CD8 +、CD28 and T cell receptor ( TCR) were detected to study the immune response. Results The CD4 +、CD8 + and TCR of group D and E were increased slightly than that of group B after 7 days, while after 14 days, those data decreased gradually and no significant difference between tissue engineered tendon and autografts ( P> 0.05), and there was significant difference between fresh allograft and tissue engineered tendon ( P< 0.05). Lymphocytes transformation induced by conA also showed no significant difference between tissue engineered tendon and autografts ( P> 0.05). Conclusion Tendon cells are hypoantigen cells, there are less secretion of soluble antigen or antigen chips dropped out from cells. Tissue engineered tendon has excellent biocompatibility.
出处
《中国修复重建外科杂志》
CAS
CSCD
2001年第2期113-117,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家重点基础研究发展规划 (973 ) :组织工程的基本科学问题 (G19990 5 43 0 8)
国家自然科学基金资助项目 (3 9870 2 0 2 )
美国
关键词
组织工程肌腱
免疫反应
T淋巴细胞亚群
肌腱损伤
修复
Tissue engineering Tendon Immune response Foundation items: National Basic Science Research and Development Grants (G1999054308)
National Natural Science Foundation of China(39870202)
China Medical Board of New York (CMB 98-682)
