摘要
目的 :对自行研制的天然脱细胞基质 (NECM)进行一系列生物相容性试验 ,评价其组织相容性。方法 :采用细胞毒性试验、全身毒性试验、溶血试验、热原试验、淋巴细胞转化试验及异体体内植入试验来评价其生物相容性。结果 :自制NECM支架材料无细胞毒性 ,无溶血现象 ,无全身毒性 ,无致热源作用 ,无致敏淋巴细胞作用 ,体内埋植不引起炎症反应。结论 :自制NECM具有良好的生物相容性 ,有可能成为理想的天然生物支架材料。
Objective:Scaffold biomaterials for cell culture of tissue engineering demand satisfactory biocompatibility.This experiment aimed to evaluate biocompatibility of self designed scaffold biomaterial of NECM.Method:The biocompatibility of self designed NECM was evaluated both in vivo and in vitro,including cytotoxic test, acute and subacute systemic toxicity test, hemolysis test, pyrogenic test, lymphocyte transformation test and long term subcutaneous implantation test of NECM.Result:Self designed NECM scaffold biomaterial for cell culture has no cytotoxicity, no acute and subacute systemic toxicity, and it did not cause inflammatory reaction.Conclusion:Self designed NECM has good biocompatibility and may become an ideal biological scaffold material for cell culture of tissue engineering.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2004年第6期363-366,共4页
Journal of Clinical Otorhinolaryngology
基金
国家杰出青年科学基金资助项目 (No:3992 5 0 35 )
国家自然科学基金资助项目 (No:396 70 781
30 0 70 80 9)
关键词
细胞外基质
生物相容性
组织工程
支架
Extracellular matrix
Biocompatibility
Tissue engineer
Stents
