摘要
目的:观察苦豆肾茶方对免疫性前列腺炎大鼠前列腺组织ICAM-1,INF-γ,MCP-1的影响。方法:采用纯化大鼠前列腺蛋白结合免疫佐剂法于第1,28天在大鼠背部多点注射建立实验性自身免疫性前列腺炎大鼠模型,并随机分为模型组、苦豆肾茶方高、低剂量组、前列泰组,另取10只大鼠背部皮下多点注射生理盐水,作为空白对照组。造模第2天开始喂药,10 mL·kg-1,苦豆肾茶方高、低剂量组灌胃苦豆肾茶方(生药9.0,4.5 g·kg-1),前列泰胶囊组灌胃前列泰(药粉0.95 g·kg-1),连续给药8周,ELISA法检测大鼠前列腺组织ICAM-1,INF-γ,MCP-1含量。结果:模型组大鼠前列腺组织ICAM-1含量较正常大鼠明显增多(P<0.05),苦豆肾茶方高剂量组可明显减少ICAM-1的生成(P<0.05);模型组大鼠前列腺组织INF-γ水平与其他各组比较无明显差异;与对照组比较,模型组大鼠前列腺组织MCP-1明显增多,苦豆肾茶方低剂量组前列腺组织MCP-1明显降低(P<0.05)。结论:苦豆肾茶方通过降低大鼠前列腺组织ICAM-1,MCP-1水平,起到抗炎免疫调节作用,这或许是其治疗慢性前列腺炎的作用机制之一。
Objective： To investigate the influence of Kudou Shencha decotion on INF-γ,ICAM-1,MCP-1 levels of prostate tissue homogenate in immunity prostatitis model rats.Method： Forty Wistar male rats were divided into 5 groups randomly： Kudou Shencha decotion group with high dosage and low dosage,Qianleitai group,the model control group and normal group.The rat model of chronic nonbacterial prostatitis was established by multiple hypodermical injection of the suspension of prostatic protein purification with Freund＇s completed adjuvant.The level of intercellular adhesion molecule（ICAM-1）,interferon γ（INF-γ） and monocyte chemotactic protein-1（MCP-1） were measured by enzyme linked immunosorbent assay（ ELISA）.Result： The content of ICAM-1 and MCP-1 in the model group was higher than that of the normal group（ P 0.05）,the content of ICAM-1 was obviously decreased in Kudou Shencha decotion group with high dosage（P 0.05）,the contents of MCP-1 were all obviously decreased in Kudou Shencha decotion groups and Qianlietai group.Compared with the model group,the contents of INF-γ in all treatment groups were decreased insignificantly.Conclusion： Kudou Shencha decotion has the action of lowering the level of ICAM-1 and MCP-1,which may be one of the mechanisms of Kudou Shencha decotion in the therapy of chronic prostatitis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第10期1918-1920,共3页
China Journal of Chinese Materia Medica
